MITOISO2

Mitochondria Isolation Kit

Name: Mitochondria Isolation Kit
Brand: SIGMA

sufficient for 50 applications (2-5 x 10⁷cells), isolation of enriched mitochondrial fraction from cells

동의어(들):

Mitochondrial Extraction Kit, Mitochondrial Purification Kit

로그인조직 및 계약 가격 보기

크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC 코드:

12352200

NACRES:

NA.32

주요 문서

모든 문서 보기

기술 서비스

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도움 문의

기술 서비스

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도움 문의

Quality Level

200

사용

sufficient for 50 applications (2-5 x 10⁷cells)

기술

ELISA: suitable
western blot: suitable

배송 상태

dry ice

저장 온도

−20°C

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일반 설명

Mitochondria, a double membrane structure, is the site of most energy production in eukaryotic cells. The mitochondria isolation kit contains two buffers: one for extracting proteins for functional studies and another for profiling in proteome studies. Additionally, the kit includes a storage buffer for preserving intact mitochondria.

애플리케이션

Mitochondria Isolation Kit has been used in the isolation of mitochondria from:

Ewing′s Sarcoma Family of Tumor (ESFT) cells
blood samples of tuberculosis patients
human neuroblastoma cells and glioma cells
myotubes
HepG2 cells

특징 및 장점

The kit allows for quick and simple extraction of a concentrated portion of mitochondria from cells.
The isolated mitochondria will mostly have intact inner and outer membranes.
Can be used to extract mitochondrial proteins for proteome analysis

기타 정보

This product is for R&D use only, not for drug, household, or other uses.
Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
Use ultrapure water for the preparation of reagents.
Please refer to the product information sheet to learn more about kit components, reagents required, storage and stability, and isolation protocols.

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제품 번호

설명

SDS

C2978CelLytic^™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 10 mLSDS

P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 2.5 mLSDS

C0356Protein Extraction Reagent Type 4 1 bottleSDS

픽토그램

GHS08,GHS07,GHS09

신호어

Warning

유해 및 위험 성명서

H302,H315,H319,H351,H361fd,H411

예방조치 성명서

P202 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P308 + P313

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

Flash Point (°F)

188.6 °F - closed cup

Flash Point (°C)

87 °C - closed cup

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

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Mitochondrial outer membrane permeability change and hypersensitivity to digitonin early in staurosporine-induced apoptosis.

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D W Moss et al.

The European journal of neuroscience, 13(3), 529-538 (2001-02-13)

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At present, there is no efficient curative therapy for cancer patients with advanced metastatic disease. Targeting of antiapoptotic molecules acting on the mitochondrial apoptosis pathway could potentially augment antimetastatic effect of cytotoxic drugs. Similarly to Bcl-2 family members, beta-galactoside-binding lectin

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문서

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Mitochondrial Stress and ROS

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Mitochondria Isolation Kit