MDQ1
Imprint® Methylated DNA Quantification Kit
To measure global DNA methylation shifts from as low as 10 ng DNA
동의어(들):
DNA Methylation Quantification, DNA methylation shift
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크기 선택
보기 변경
제품정보 (DICE 배송 시 비용 별도)
NACRES:
NA.54
UNSPSC Code:
41116158
General description
Imprint™ Methylated DNA Quantification technology provides a rapid and reliable method to measure global DNA methylation shifts. The Imprint Methylated DNA Quantification Kit contains all reagents required to detect relative levels of methylated DNA. Up to 200 ng of purified DNA is bound to the wells of the assay strip. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. The amount of methylated DNA present in the sample is proportional to the absorbance measured.
Application
Imprint® Methylated DNA Quantification Kit has been used in global DNA methylation analysis.
Features and Benefits
- ELISA based format
- Procedure completes in 4 easy to follow steps
- All reagents supplied including control methylated DNA
- Detection limit is 5 ng of fully methylated DNA
- Input DNA may be as low as 10-200 ng
- Procedure completes in 3.5 hours
- 96 well format allows option of studying single samples or high-throughput studies
- Optimized antibody and reagents with high specificity to 5-mC; no cross-reactivity to unmethylated cytosine
- May be used with adherent and suspension cells, tissues, plasma and other body fluids such as saliva, urine and serum
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
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Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1
wgk
WGK 3
저장 등급
10 - Combustible liquids
프로토콜
Imprint ® Methylated DNA Quantification Kit (MDQ1) Protocol
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
문서
Global DNA Modification Kit (MDQ1) monitors DNA methylation via sandwich ELISA-like assay, aiding in epigenetic research.
Kazuhisa Nakano et al.
Journal of immunology (Baltimore, Md. : 1950), 190(3), 1297-1303 (2013-01-02)
Rheumatoid arthritis (RA) is a chronic inflammatory disease in which fibroblast-like synoviocytes (FLS) exhibit an aggressive phenotype. Although the mechanisms responsible are not well defined, epigenetic determinants such as DNA methylation might contribute. DNA methyltransferases (DNMTs) are critical enzymes that
Vitalina M Komashko et al.
Epigenetics, 5(3), 229-240 (2010-03-23)
Methylation of DNA in combination with histone modifications establishes an epigenetic code that ensures the proper control of gene expression. Although DNA methyltransferases have been shown to interact with histone methyltransferases such as EZH2 (which methylates histone H3 on lysine
INSIG2 rs7566605 single nucleotide variant and global DNA methylation index levels are associated with weight loss in a personalized weight reduction program
Pirini F, et al.
Molecular Medicine Reports, 17(1), 1699-1709 (2018)
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| MDQ1-48RXN | 04061835507009 |
| MDQ1-96RXN | 04061835507016 |