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M3148

2-Mercaptoethanol

Name: 2-Mercaptoethanol
Brand: SIGMA

Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

동의어(들):

β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

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제품정보 (DICE 배송 시 비용 별도)

Linear Formula:

HSCH₂CH₂OH

CAS 번호:

60-24-2

Molecular Weight:

78.13

UNSPSC Code:

12161504

NACRES:

NA.31

PubChem Substance ID:

24896800

EC Number:

200-464-6

Beilstein/REAXYS Number:

773648

MDL number:

MFCD00004890

기술 서비스

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도움 문의

속성

grade

Molecular Biology

vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

product line

BioReagent

assay

99% (GC/titration)

form

liquid

expl. lim.

18 %

reaction suitability

reagent type: reductant

concentration

14.3 M (pure liquid)

technique(s)

cell culture | mammalian: suitable, electrophoresis: suitable

refractive index

n20/D 1.500 (lit.)

pH

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

solubility

H₂O: soluble 1 mL/mL

density

1.114 g/mL at 25 °C (lit.)

foreign activity

DNase, RNase, protease, none detected

storage temp.

2-8°C

SMILES string

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

설명

Application

Reducing agent
β-mercaptoethanol or 2-mercaptoethanol has been used:

as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line)
to lyse NuLi-1 (Normal Lung, University of Iowa-1) cells
to trypsinize mouse embryonic stem cells

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

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pictograms

GHS06,GHS08,GHS05,GHS09

signalword

Danger

hcodes

H301 + H331,H310,H315,H317,H318,H361f,H373,H410

pcodes

P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

target_organs

Liver,Heart

저장 등급

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

flash_point_f

165.2 °F - closed cup

flash_point_c

74 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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국제 무역 품목 번호

SKU	GTIN
M3148-.15ML	04061838080097
M3148-.9ML	04061838243348
M3148-25ML	04061838258496
M3148-2ML	04061838243355
M3148-100ML	04061838258472
M3148-250ML	04061838258489
M3148-500ML	04061834046424