L8511
Leupeptin
powder, ≥85% (HPLC)
동의어(들):
Leupeptin hemisulfate salt, Acetyl-Leu-Leu-Arg-al, N-Acetyl-L-leucyl-L-leucyl-L-argininal hemisulfate salt
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제품정보 (DICE 배송 시 비용 별도)
Linear Formula:
C20H38N6O4 · 1/2H2SO4
CAS 번호:
Molecular Weight:
475.59
UNSPSC Code:
12352202
PubChem Substance ID:
NACRES:
NA.77
MDL number:
제품 이름
Leupeptin, synthetic, ≥85% (HPLC)
biological source
synthetic
Quality Level
assay
≥85% (HPLC)
form
powder
solubility
H2O: 50 mg/mL
storage temp.
−20°C
SMILES string
OS(O)(=O)=O.CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C=O.CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C=O
InChI
1S/2C20H38N6O4.H2O4S/c2*1-12(2)9-16(24-14(5)28)19(30)26-17(10-13(3)4)18(29)25-15(11-27)7-6-8-23-20(21)22;1-5(2,3)4/h2*11-13,15-17H,6-10H2,1-5H3,(H,24,28)(H,25,29)(H,26,30)(H4,21,22,23);(H2,1,2,3,4)/t2*15-,16-,17-;/m00./s1
InChI key
CIPMKIHUGVGQTG-VFFZMTJFSA-N
Application
Leupeptin has been used as a protease inhibitor: in ice-cold lysis buffer to harvest cells for western immunoblotting and immunoprecipitation,in chromatin immunoprecipitation (ChIP) lysis buffer for the isolation of fragmented chromatin samples in lysis buffer I to lyse the cells for tandem affinity purification (TAP)
Biochem/physiol Actions
Leupeptin helps to protect motoneurons from apoptosis. It also helps to enhance muscle function following nerve injury. It possesses neurotrophic action and has a growth-stimulating effect.
Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain, calpain, and cathepsin B. Does not inhibit pepsin, cathepsins A and D, thrombin, or α-chymotrypsin. Effective concentration 10-100 μM.
Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain, calpain, and cathepsin B. Does not inhibit pepsin, cathepsins A and D, thrombin, or α-chymotrypsin. Effective concentration 10-100 μM. There have been numerous studies using leupeptin to protect against hearing loss caused by acoustic overstimulation or the ototoxic antibiotic gentamicin. (Loss of cochlear hair cells is believed to be mediated by calpain.)
Analysis Note
Leupeptin gives multiple peaks on HPLC due to equilibria among three forms in solution. Purity determined using three main peaks. Majority of contaminating peptide is racemized leupeptin.
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
LAMTOR/Ragulator is a negative regulator of Arl8b-and BORC-dependent late endosomal positioning
Filipek PA, et al.
The Journal of Cell Biology, 216(12), 4199-4215 (2017)
An Improved Method for Measuring Chromatin-binding Dynamics Using Time-dependent Formaldehyde Crosslinking
Hoffman EA, et al.
Bio-protocol, 8(4) (2018)
Inhibition of calpains, by treatment with leupeptin, improves motoneuron survival and muscle function in models of motoneuron degeneration
Kieran D and Greensmith L
Neuroscience, 125(2), 427-439 (2004)
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| L8511-250MG | 04061833263662 |
| L8511-25MG | 04061833263679 |
| L8511-50MG | 04061833263686 |
| L8511-100MG | 04061833966044 |
| L8511-10MG | 04061833621332 |
| L8511-5MG | 04061833966051 |