grade
Molecular Biology
Quality Level
sterility
non-sterile
form
powder
composition
Agar, 15 g/L , NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L
technique(s)
microbiological culture: suitable
application(s)
agriculture
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.
Application
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
Features and Benefits
Lennox EZMix powder with agar provides:
- A budget-friendly alternative to pre-poured plates
- Granulated, dust-free format for safer handling and faster mixing
- Convenient small package to eliminate weighing
- Easy scale-up using larger package sizes
- Standard formulation
Preparation Note
1. Suspend 35.6 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1 M magnesium sulfate after autoclaving.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1 M magnesium sulfate after autoclaving.
Add 35.6g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.
Legal Information
Zeocin is a registered trademark of Cayla Sarl
저장 등급
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
프로토콜
Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.
General protocols for growth of competent cells in microbial medium.
문서
General protocols for growth of competent cells and their transformation (uptake of DNA).
Sambrook, J., et al
Molecular Cloning: A Laboratory Manual, 2, 1-1 (1989)
Ausubel, F. M.
Current Protocols in Molecular Biology, 1, 3-3 (1994)
Alexis Kaushansky et al.
Nature protocols, 5(4), 773-790 (2010-04-03)
Protein microarrays provide an efficient way to identify and quantify protein-protein interactions in high throughput. One drawback of this technique is that proteins show a broad range of physicochemical properties and are often difficult to produce recombinantly. To circumvent these
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| L7533-1KG | 04061833965559 |
| L7533-6X500ML | 04061833965566 |