storage temp.
−20°C
General description
Overview
Compatible with any thermal cycler, KiCqStart Primers are ready-to-order, pre-designed primer pairs for quantifying gene expression by SYBR Green I RT-qPCR (Reverse Transcription Quantitative Real-Time PCR). To comply with MIQE, they have been developed using sophisticated bioinformatics tools and validated in silico to avoid off-target amplification1. KiCqStart Primers are available as up to three ranked sets of forward and reverse primer pairs for all genes from common model organisms.
1SNP loci have been avoided during the design process when possible. Please consult dbSNP to determine if your gene has unavoidable SNPs.
To learn more about KiCqStart Primers visit our Frequently Asked Questions section.
Specifications
Design characteristics
Oligo characteristics
Sequences are provided at the time of shipment.
295% of primer pairs fall within these specifications
Rank
Each gene has up to three alternative primer pairs, which are identified as follows:
Compatible with any thermal cycler, KiCqStart Primers are ready-to-order, pre-designed primer pairs for quantifying gene expression by SYBR Green I RT-qPCR (Reverse Transcription Quantitative Real-Time PCR). To comply with MIQE, they have been developed using sophisticated bioinformatics tools and validated in silico to avoid off-target amplification1. KiCqStart Primers are available as up to three ranked sets of forward and reverse primer pairs for all genes from common model organisms.
1SNP loci have been avoided during the design process when possible. Please consult dbSNP to determine if your gene has unavoidable SNPs.
To learn more about KiCqStart Primers visit our Frequently Asked Questions section.
Specifications
Design characteristics
- GC Content: 20 - 80%2
- Melting Temperature: 55.0 ± 5 ºC
- Secondary Structure: None
- Excessive 3′ Clamping: Minimal
- Homology: None
Oligo characteristics
- Molecule: DNA (all sequences verified by mass spec QC); no modifications
- Contents: 1 forward and 1 reverse primer
- Length: 18 - 24 bases2
- Purification: RP cartridge
- Format: 3 OD minimum yield, dry; 1 oligonucleotide per tube
Sequences are provided at the time of shipment.
295% of primer pairs fall within these specifications
Rank
Each gene has up to three alternative primer pairs, which are identified as follows:
- 1: The middle of one primer overlaps an exon splice site
- 2: The 5′ or 3′ end of one primer overlaps an exon splice site
- 3: The amplicon, but neither primer, overlaps an exon splice site
- 4: Primer overlap is not possible for various reasons, e.g. the gene has only one exon (primer pairs with this rank may lead to co-amplification of genomic DNA).
Properties include:
- Available species: human, mouse, rat, zebrafish, cow, horse, Arabidopsis, chicken, dog, rabbit, pig, cat, rice, soybean, rhesus, Drosophila, C. elegans, corn, X. laevis, Guinea Pig, and Chinese Hamster.
- Components: two primers, one oligo per tube
- Shipping condition: dry, ambient temperature
- Storage condition: dry or wet single-use aliquots
Application
Product use: gene expression analysis
Features and Benefits
KiCqStart Primers are sensitive, specific, and will save you time and money by eliminating the tedious steps required to develop an effective SYBR Green I RT-qPCR Assay.
Benefits include:
Benefits include:
- Target analysis is done
- Design characteristics are set
- Oligo characteristics are set
- Easy to order
Other Notes
Frequently Asked Questions section is available for this product.
Legal Information
KiCqStart is a registered trademark of QIAGEN Beverly Inc.
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries
문서
Primer pairs detect prevalent eukaryotic genes, but may co-amplify other transcripts, emphasizing the importance of comprehensive primer design.
Mara Ribeiro Almeida et al.
Molecular nutrition & food research, 60(7), 1615-1624 (2016-03-05)
Vitamin B6 plays crucial roles on brain development and its maternal deficiency impacts the gamma-aminobutyric acid (GABA)ergic, serotonergic, glutamatergic, and dopaminergic systems in offspring. However, the molecular mechanisms underlying these neurological changes are not well understood. Thus, we aimed at
Agata Nyga et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 107(5), 1352-1362 (2018-09-28)
We aimed to find a biomarker for patients with adverse reaction to metal debris (ARMD) due to a metal-on-metal (MoM) hip implant. First, we compared molecular markers of hypoxia-inducible factor (HIF) pathway activation (BNIP3, GLUT1, HO1, VEGF, and HIF1A) and
Helen Heath et al.
The EMBO journal, 27(21), 2839-2850 (2008-10-17)
The 11-zinc finger protein CCCTC-binding factor (CTCF) is a highly conserved protein, involved in imprinting, long-range chromatin interactions and transcription. To investigate its function in vivo, we generated mice with a conditional Ctcf knockout allele. Consistent with a previous report