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Merck

I8896

Sigma-Aldrich

IGEPAL® CA-630

Molecular Biology

동의어(들):

Octylphenoxy poly(ethyleneoxy)ethanol, branched

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제품정보 (DICE 배송 시 비용 별도)

Linear Formula:
(C2H4O)nC14H22O
CAS 번호:
UNSPSC 코드:
12352104
NACRES:
NA.31
기술 서비스
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도움 문의

Quality Level

Grade

Molecular Biology

설명

non-ionic

양식

liquid

기술

IR spectroscopy: suitable

CMC

0.08 mM (20-25°C)

점도

~250-350 cP(25 °C)

mp

6 °C

solubility

water: soluble at 20 °C

density

1.060 g/cm3

HLB

13

외래 활성

DNase and RNase, none detected

SMILES string

O(CCO)c1ccc(cc1)C(CC(C)(C)C)(C)C

InChI

1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3

InChI key

JYCQQPHGFMYQCF-UHFFFAOYSA-N

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일반 설명

IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes.It is chemically indistinguishable from Nonidet P-40.

애플리케이션

IGEPAL® CA-630 has been used
  • as neutral detergent in homogenizing buffer during western blotting of mouse muscle homogenates
  • In X-gal staining for studying the lacZ expression in E8.5-E10.5 embryos
  • In Cell-Lysis (CL) buffer for preparing RT-qPCR-ready cell lysates

분석 메모

Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.

기타 정보

Nonionic, non-denaturing detergent

법적 정보

IGEPAL is a registered trademark of Solvay

면책조항

May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.

픽토그램

CorrosionExclamation markEnvironment

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

483.8 °F - closed cup

Flash Point (°C)

251 °C - closed cup

개인 보호 장비

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Amina Abbadi et al.
The Journal of biological chemistry, 291(3), 1448-1455 (2015-11-26)
Many cells, including murine airway epithelial cells, respond to a variety of inflammatory stimuli by synthesizing leukocyte-adhesive hyaluronan (HA) cables that remain attached to their cell surfaces. This study shows that air-liquid interface cultures of murine airway epithelial cells (AECs)
Kenneth Shatzkes et al.
Scientific reports, 4, 4659-4659 (2014-04-12)
Sample nucleic acid purification can often be rate-limiting for conventional quantitative PCR (qPCR) workflows. We recently developed high-throughput virus microneutralization assays using an endpoint assessment approach based on reverse transcription qPCR (RT-qPCR). The need for cumbersome RNA purification is circumvented
Bojana Lucic et al.
Nature communications, 10(1), 4059-4059 (2019-09-08)
HIV-1 recurrently targets active genes and integrates in the proximity of the nuclear pore compartment in CD4+ T cells. However, the genomic features of these genes and the relevance of their transcriptional activity for HIV-1 integration have so far remained
Soichiro Yamanaka et al.
Developmental cell, 51(1), 21-34 (2019-09-03)
Facultative heterochromatin forms and reorganizes in response to external stimuli. However, how the initial establishment of such a chromatin state is regulated in cell-cycle-arrested cells remains unexplored. Mouse gonocytes are arrested male germ cells, at which stage the genome-wide DNA
Joseph A Roche et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 59(11), 964-975 (2011-11-02)
Mutations in the DYSF gene that severely reduce the levels of the protein dysferlin are implicated in muscle-wasting syndromes known as dysferlinopathies. Although studies of its function in skeletal muscle have focused on its potential role in repairing the plasma

프로토콜

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

관련 콘텐츠

친화성, GST 풀다운, TAP, 공동 면역 침전법을 활용한 풀다운 분석을 통해 in vitro 단백질-단백질 상호작용을 분석합니다.

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

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