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Merck

I3786

Protease Inhibitor Cocktail

Animal-Free, DMSO solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use with mammalian cell and tissue extract, DMSO solution

동의어(들):

Protease inhibitor

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UNSPSC Code:
12352200
NACRES:
NA.77
Form:
DMSO solution
Solubility:
water: soluble
Storage temp.:
−20°C
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제품 이름

Protease Inhibitor Cocktail, Animal Component Free, for use with mammalian cell and tissue extract, DMSO solution

Quality Level

form

DMSO solution

solubility

water: soluble

storage temp.

−20°C

General description

Components of this cocktail are of non-animal origin and no animal products were used in the component production process. All vessels and instruments used for the cocktail production are dedicated for animal component free production and have never encountered an animal product. Aprotinin used in this cocktail is a recombinant bovine protein expressed in plants (Nicotiana).
This protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts.

Application

Animal component free (ACF). Optimized and tested for general use. Can also be used for purification of histidine-tagged proteins, as chelating agents have been omitted.

Biochem/physiol Actions

Inhibits serine, cysteine, aspartic proteases, and aminopeptidases.

Physical form

The components in this ACF protease inhibitor cocktail do not originate from animal sources and can thus be used in the production of animal-free proteins.
This ACF cocktail is supplied as a ready-to-use solution in DMSO. One ml of the cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g (wet weight) of bovine liver, or 10 mL of cell lysate obtained from CHO cells at a cell density of 108 cells per mL.

Other Notes

AEBSF
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A


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Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

저장 등급

10 - Combustible liquids

wgk

WGK 2

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup



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관련 콘텐츠

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.


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SKUGTIN
I3786-1ML04061833856376
I3786-5ML04061833856383