H6254
Hyaluronidase from sheep testes
Type V, lyophilized powder, ≥1,500 units/mg solid
동의어(들):
Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
로그인조직 및 계약 가격 보기
크기 선택
제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54
생물학적 소스
sheep testis
Quality Level
유형
Type V
양식
lyophilized powder
특이 활성도
≥1,500 units/mg solid
분자량
55 kDa
응용 분야
diagnostic assay manufacturing
저장 온도
−20°C
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일반 설명
Hyaluronidase is present in high amount in testis as it aids fertilization of the mammalian egg. Mammalian hyaluronidase has a catalytic epidermal growth factor (EGF)-like domain and a C-terminal cysteine rich region.
애플리케이션
Hyaluronidase has been used:
- as a component of enzyme mix for the isolation of sertoli and germ cells from seminiferous tubules
- in the pre-treatment of deparaffinized adenomas sections for immunohistochemistry
- in the digestion of umbilical cord blood vessel for the generation of umbilical cord stromal stem cell lines
생화학적/생리학적 작용
Hyaluronidase from sheep testis is commercially used to depolymerise hyaluronic acid and chondroitin-sulphate in connective tissue and cartilage respectively.
These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.
단위 정의
One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
기타 정보
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
Human umbilical cord stromal stem cell express CD10 and exert contractile properties
Farias VA, et al.
Placenta, 32(1), 86-95 (2011)
Structure of human hyaluronidase-1, a hyaluronan hydrolyzing enzyme involved in tumor growth and angiogenesis
Chao KL, et al.
Biochemistry, 46(23), 6911-6920 (2007)
Yan Liang et al.
Scientific reports, 7(1), 12148-12148 (2017-09-25)
Meniscus fibrochondrocytes (MFCs) may be the optimal cell source to repair non-healing meniscus injuries using tissue engineering strategies. In this study, we investigated the effects of mitotic divisions and oxygen tension on the plasticity of adult human MFCs. Our assessment
Cartilage-specific matrix protein chondromodulin-I is associated with chondroid formation in salivary pleomorphic adenomas: immunohistochemical analysis
Kusafuka K, et al.
The American Journal of Pathology, 158(4), 1465-1472 (2001)
Isolation of Sertoli cells from adult rat testes: an approach to ex vivo studies of Sertoli cell function
Anway MD, et al.
Biology of Reproduction, 68(3), 996-1002 (2003)
문서
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
프로토콜
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
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