H6147
HEPES
BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)
동의어(들):
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
크기 선택
보기 변경
제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
C8H18N2O4S
CAS 번호:
Molecular Weight:
238.30
UNSPSC Code:
12161704
NACRES:
NA.75
PubChem Substance ID:
EC Number:
230-907-9
Beilstein/REAXYS Number:
883043
MDL number:
product line
BioXtra
Quality Level
assay
≥99.5% (titration)
form
powder
technique(s)
cell culture | embryo: suitable
pH
5.0-6.5 (25 °C, 238 g/L)
useful pH range
6.8-8.2
pKa (25 °C)
7.5
solubility
water: 500 mg/mL, clear, colorless
absorption
≤0.05 at 290 in H2O at 33%
SMILES string
OCCN1CCN(CC1)CCS(O)(=O)=O
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
General description
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. It is typically used in cell culture at concentration between 5mM to 30mM.
Useful pH range: 6.8 - 8.2
Application
HEPES has been used:
- As a constituent of modified Eagle′s minimum essential medium for in vitro culturing of spermatogenic cells and as a constituent of the cryoprotectant used to preserve these germ cells
- As a component of modified Ham′s F-10 for the incubation of doe zygotes to carry out Haploid male hemizygous recipient production
- in the preparation of 90% Percoll solution used in Percoll- gradient method for sperm collection and preparation
Other Notes
Easily compare specifications for HEPES products with the HEPES specification table.
This product has been qualified for use in cell and mouse embryo culture.
저장 등급
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
문서
Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
관련 콘텐츠
HEPES Specification Comparison
Short-term in-vitro culture and cryopreservation of spermatogenic cells used for human in-vitro conception.
Aslam I and Fishel S
Human Reproduction, 13(3), 634-638 (1998)
The effects of L-ergothioneine and L-ascorbic acid on the in vitro maturation (IVM) and embryonic development (IVC) of sheep oocytes.
Pancarci SM, et al.
Kafkas U?niversitesi veteriner faku?ltesi dergisi, 16, 757-763 (2010)
Viable offspring derived from cryopreserved haploid rabbit parthenotes.
Garci'a-Ximenez F and Escriba MJ
Theriogenology, 57(4), 1319-1325 (2002)
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| H6147-100G | 04061833800379 |
| H6147-25G | 04061833800393 |
| H6147-500G | 04061833800416 |