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H3537

HEPES solution

Name: HEPES solution
Brand: SIGMA

99.5%, liquid, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

동의어(들):

N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

조직 및 계약 가격을 보려면 로그인를 클릭합니다.

크기 선택

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제품정보 (DICE 배송 시 비용 별도)

CAS 번호:

7365-45-9

UNSPSC Code:

12161700

PubChem Substance ID:

329815199

NACRES:

NA.25

MDL number:

MFCD00006158

기술 서비스

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속성

제품 이름

HEPES solution, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

grade

BioPerformance Certified

Quality Level

200

sterility

0.2 μm filtered

form

liquid

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

Bioburden, tested, DNase, RNase, Protease, Nickase, free, endotoxin, tested

pH

5.0-6.0

useful pH range

6.8-8.2

cation traces

Fe: <5 ppm, heavy metals (as Pb): <5 ppm

suitability

suitable for molecular biology

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

설명

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

RNAse HEPES has been used:

To supplement Dulbecco′smodified Eagle′smedium for maintenance of cell line and RPMI medium to wash rat islets
To recover purified ribonucleotide
As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing
As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets

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저장 등급

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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국제 무역 품목 번호

SKU	GTIN
H3537-100ML	04061833793435
H3537-1L	04061835545476
H3537-500ML	04061833793442