Gamborg′s B-5 Basal Medium with Minimal Organics

Gamborg′s B-5 basal medium is an efficient nutrient mixture for plants.

Gamborg′ B-5 medium may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro culture of plant cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.

Gamborg′s B-5 Basal Medium with Minimal Organics has also been used:

• as a component of liquid media for the tissue culture of Brassica rapa
• as a component of callus inducing medium for culturing Arabidopsis cardiolipin mutant seedlings
• as a component of solid GM medium for germination of Arabidopsis seeds

Formulated to contain 3.2 grams of powder per liter of medium.

With the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968).

Media Formulation