G2174
β-Glucuronidase from limpets (Patella vulgata)
aqueous solution, ≥85,000 units/mL
동의어(들):
β-D-Glucuronide glucuronosohydrolase
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크기 선택
보기 변경
제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥85,000 units/mL
Biological source:
limpet (Patella vulgata)
biological source
limpet (Patella vulgata)
Quality Level
form
aqueous solution
specific activity
≥85,000 units/mL
secondary activity
≤7,500 units/mL sulfatase
storage temp.
2-8°C
Application
New Technical Article Comparing Performance of Different Enzymes
Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
The enzyme from patella vulgata is reported to be more effective in hydrolyzing opioid-glucuronides than the Helix pomatia, bovine liver and E. coli enzymes
Features and Benefits
Save time with no reconstitution in this convenient liquid format.
Physical form
Aqueous solution in 0.9% NaCl with 0.02% sodium azide as preservative.
Other Notes
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
저장 등급
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
프로토콜
Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.
-THC solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material
문서
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
관련 콘텐츠
Instructions
J Combie et al.
Clinical chemistry, 28(1), 83-86 (1982-01-01)
beta-Glucuronidase from Patella vulgata, Helix aspersa, Helix pomatia, and bovine liver were evaluated for usefulness in routine hydrolysis of drug-glucuronic acid conjugates from equine urine samples. Factors affecting the reaction rate (enzyme concentration, ligand concentration, temperature, and pH) were optimized.
T A Jennison et al.
Journal of analytical toxicology, 17(4), 208-210 (1993-07-01)
Methods to confirm morphine in urine require hydrolysis to liberate morphine from its 3-beta-D glucuronide (M-3G) conjugate. Lengthy enzyme hydrolysis procedures prolong testing turnaround time whereas rapid enzyme methods may produce a low conversion of M-3G to morphine. The purpose
Victor Aguilar-Hernández et al.
Plant molecular biology, 84(4-5), 429-441 (2013-10-19)
Gene duplication events exert key functions on gene innovations during the evolution of the eukaryotic genomes. A large portion of the total gene content in plants arose from tandem duplications events, which often result in paralog genes with high sequence
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| G2174-1ML | 04061838087447 |
| G2174-10ML | 04061838087430 |
| G2174-25ML | 04061833628737 |
| G2174-5ML | 04061838087454 |