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G1767

Glycogen from mussel, Mytilus genus

Name: Glycogen from mussel, Mytilus genus
Brand: SIGMA

for DNA precipitations

동의어(들):

DNA and RNA precipitation

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제품정보 (DICE 배송 시 비용 별도)

CAS 번호:

9005-79-2

UNSPSC Code:

12352200

NACRES:

NA.52

EC Number:

232-683-8

MDL number:

MFCD23701213

기술 서비스

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도움 문의

속성

grade

Molecular Biology

Quality Level

200

form

liquid

packaging

vial of 1 mL

concentration

19-22 mg/mL

storage temp.

−20°C

SMILES string

O1[C@@H]([C@@H]([C@H]([C@@H]([C@H]1CO[C@H]4O[C@@H]([C@H]([C@@H]([C@H]4O)O)O)CO)O[C@H]3O[C@@H]([C@H]([C@@H]([C@H]3O)O)O)CO)O)O)O[C@@H]2[C@H](O[C@@H]([C@@H]([C@H]2O)O)O)CO

InChI

1S/C24H42O21/c25-1-5-9(28)11(30)16(35)22(41-5)39-4-8-20(45-23-17(36)12(31)10(29)6(2-26)42-23)14(33)18(37)24(43-8)44-19-7(3-27)40-21(38)15(34)13(19)32/h5-38H,1-4H2/t5-,6-,7-,8-,9-,10-,11+,12+,13-,14-,15-,16-,17-,18-,19-,20-,21+,22+,23-,24-/m1/s1

InChI key

BYSGBSNPRWKUQH-UJDJLXLFSA-N

설명

General description

Glycogen is a branched polymer of glucose synthesized by animal cells for energy storage and release. It is constructed of predominantly α1→4 glycosidic bonds with branches created through α1→6 glycosidic bonds.

Glycogen is a branched polymer of glucose that coprecipitates nucleic acids during isolation and purification.

Application

Glycogen from Mytilus genus (Blue mussel) has been used

as a coprecipitant in the isolation of RNA from hepatocytes and yeast cells.
as a reference standard in calibration curve generation to quantify hepatic glycogen content.
as a carrier molecule for DNA and RNA, replacing tRNA or sonicated DNA
for the precipitation of DNA in the presence of ammonium acetate and ethanol or isopropanol
for recovery of tRNA, yeast RNA, sonicated DNA and oligonucleotides as short as 8 base pairs

Biochem/physiol Actions

Glycogen levels in Mytilus genus shows variation during seasonal changes. It is a crucial stored energy reserve for Mussels and is utilized during gametogenesis. Glycogen levels dictate the reproductive capacity and development of mussels larva. Glycogen aids in the coprecipitation of nucleic acids during isolation and purification.

Glycogen is an inert carrier that traps nucleic acids. It forms a precipitate with DNA/RNA while being insoluble in ethanol solution. Due to the manufacturing process, there is low risk of contaminating nucleic acids in the glycogen. This product is tested for DNase, RNase, and NICKase, but not nucleic acids.

Features and Benefits

product provided is 1 mL total volume
precipitates even small amounts of DNA and RNA present in dilute solutions
does not interfere with OD 260/280 readings

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저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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국제 무역 품목 번호

SKU	GTIN
G1767-1VL	04061835573035