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Merck

FG0465

IMDM

With stable-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

동의어(들):

Cell culture medium, Iscove′s MDM, Iscove’s Modified Dulbecco’s Medium, Modified Dulbecco′s medium

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UNSPSC Code:
12352207
NACRES:
NA.71
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제품 이름

Iscove′s Modified Dulbecco′s Medium, With L-alanyl-L-glutamine and sodium bicarbonate, sterile-filtered, liquid, suitable for cell culture

Quality Level

sterility

sterile-filtered, sterile

product line

AQmedia

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

components

stable glutamine: yes
NaHCO3: yes
glucose: yes
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

General description

This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

Application

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco¢s Modified Eagle¢s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove¢s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Legal Information

AQmedia is a trademark of Sigma-Aldrich Co. LLC


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저장 등급

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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