General description
Enzymatic protein deglycosylation kit contains all the enzymes and reagents required for the complete removal of N-linked and simple O-linked carbohydrates from glycoproteins. This kit is employed to cleave the complex Core 2 O-linked carbohydrates including those containing polylactosamine.
Application
Enzymatic Protein Deglycosylation Kit has been used for the deglycosylation of various enzymes.
Enzymatic protein deglycosylation kit has been used in:
- protein deglycosylation
- deglycosylation of tissue factor pathway inhibitor (TFPI) α and β
- deglycosylation of native exo-β-(1,3)-glucanase EXG1
Features and Benefits
Enzymatic protein deglycosylation kit has the following features:
- two or more mg of glycoprotein is deglycosylated
- single reaction at neutral pH
- native & denaturing procedures
- no degradation of protein
- O-linked sugars containing polysialic acid are eliminated
- control glycoprotein provided
키트 구성품 전용
제품 번호
설명
- PNGase F 1 mL/vial
- O-Glycosidase 20 μL
- α-2(3,6,8,9)-Neuraminidase 20 μL
- Fetuin Control .5 mg
- 5× Reaction Buffer .2 mL
- Denaturation Solution .1 mL
- Triton™ X-100 .1 mL
모두 보기 (9)
signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
저장 등급
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
문서
Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.
Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
Protein modifications are crucial for disease study. Analysis methods are key.
관련 콘텐츠
Glycobiology and Glycoproteomics Brochure
Guillain-Barre syndrome: First description of a snake envenomation aetiology
Neil J, et al.
Journal of Neuroimmunology, 242(1-2), 72-77 (2012)
Julien Chapuis et al.
Human molecular genetics, 20(10), 2026-2036 (2011-03-02)
Alzheimer's disease (AD), the most common neurodegenerative disorder, is characterized by cerebral deposition of amyloid-β (Aβ), a series of peptides derived from the processing of the amyloid-β precursor protein (APP). To identify new candidate genes for AD, we recently performed
Angela Mika et al.
PLoS neglected tropical diseases, 5(9), e1331-e1331 (2011-10-08)
Pruritic scabies lesions caused by Sarcoptes scabiei burrowing in the stratum corneum of human skin facilitate opportunistic bacterial infections. Emerging resistance to current therapeutics emphasizes the need to identify novel targets for protective intervention. We have characterized several protein families
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| EDEGLY-1KT | 04061833609392 |