E4274
Endotoxin Removal solution
Remove lipopolysaccharides (LPS) from plasmid DNA preparations
동의어(들):
ET removal buffer, ET removal reagent, ET removal solution, endo removal buffer, endo removal reagent, endo removal solution, endotoxin contamination removal, endotoxin removal buffer, endotoxin removal reagent, plasmid purification endo, plasmid purification endo free
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크기 선택
보기 변경
제품정보 (DICE 배송 시 비용 별도)
NACRES:
NA.52
UNSPSC Code:
12352200
Quality Level
Application
Endotoxin Removal solution has been used to remove lipopolysaccharide (LPS) from Baker′s yeast RNA.
Biochem/physiol Actions
The reagent relies on the micellar properties of Triton X-114 to remove endotoxins from solution. When warmed above 20 °C, micelles aggregate into a separate phase that sequesters amphiphilic solutes, including lipopolysaccharides. The hydrophilic DNA remains in the aqueous phase.
Preparation Note
1 volume treats 5 volumes of DNA solution (≤1mg/ml)
저장 등급
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
문서
균체 내독소란 무엇입니까? 체외 세포 배양에서의 균체 내독소 오염에 관한 자주 묻는 질문입니다. LAL 분석을 사용한 내독소 검사 방법, 실험실 내독소 오염의 공통 출처와 세포 라인을 배양할 때 내독소 오염을 피하는 방법에 대한 자세한 내용입니다.
FAQs on bacterial endotoxin contamination, details on endotoxin testing using the LAL assay, and tips to avoid contamination in cell cultures.
관련 콘텐츠
Determination of endotoxin through an aptamer-based impedance biosensor.
Su W
Biosensors And Bioelectronics, 32(1), 32-36 (2012)
M Cotten et al.
Gene therapy, 1(4), 239-246 (1994-07-01)
Endotoxin (lipopolysaccharide, LPS) is commonly found as a contaminant in plasmid DNA preparations. We demonstrate here that the quantities of LPS typically contaminating DNA preparations can generate a toxicity to primary cells (primary human skin fibroblasts, primary human melanoma cells)
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| E4274-25ML | 04061833603598 |
| E4274-45ML | 04061837298622 |
| E4274-60ML | 04061837298646 |