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Merck

D5648

DMEM - high glucose

With ʟ-glutamine, without sodium bicarbonate and sodium pyruvate, powder, suitable for cell culture

동의어(들):

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352207
NACRES:
NA.75
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제품 이름

Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

components

phenol red: yes
L-glutamine: yes
NaHCO3: no
HEPES: no
glucose: high
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
Dulbecco′s Modified Eagle′s Medium - high glucose has been used as the culture medium for various cell types including the human cervical endocervical epithelial End1/E6E7 cells, human adenocarcinoma epithelial cells (HeLa), human alveolar adenocarcinoma epithelial cells (A549), human osteosarcoma epithelial cells, human diploid cell line , primary hepatocytes and synovial tissue

Preparation Note

Formulated to contain 13.4 grams of powder per liter of medium.
Supplement with 3.7 g/L sodium bicarbonate.


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저장 등급

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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시험 성적서(COA)

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문서 라이브러리 방문


문서

Cell culture protocol for passaging and splitting suspension cell lines

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국제 무역 품목 번호

SKUGTIN
D5648-10L04061833586532
D5648-25KG04061834408376
D5648-10X1L04061835518951
D5648-50L04061833586549