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Merck

D1806

Sigma-Aldrich

Taq DNA Polymerase from Thermus aquaticus

with 10× PCR reaction buffer containing MgCl2

동의어(들):

MgCl2 taq polymerase, Taq polymerase

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

CAS 번호:
9012-90-2
EC 번호:
2.7.7.7 (BRENDA, IUBMB)
MDL number:
MFCD00166026
UNSPSC 코드:
12352204
NACRES:
NA.55

주요 문서

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기술 서비스
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도움 문의

생물학적 소스

enzyme from bacterial (Thermus Aquaticus)

Quality Level

200

재조합

expressed in E. coli

양식

liquid

사용

sufficient for 10000 reactions
 sufficient for 3000 reactions
 sufficient for 500 reactions

분자량

94 kDa

특징

dNTPs included: no
hotstart: no

농도

5 units/μL

기술

PCR: suitable

색상

colorless

입력

purified DNA

적합성

suitable for PCR and automated sequencing reactions

응용 분야

agriculture

배송 상태

wet ice

저장 온도

−20°C

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관련 카테고리

일반 설명

Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. The enzyme is in a recombinant form, expressed in E. coli. It can withstand repeated heating to 95 °C without significant loss of activity. Each lot of Taq DNA Polymerase is tested for PCR amplification and double-stranded sequencing.

애플리케이션

Taq DNA Polymerase from Thermus aquaticus has been used:
  • in the quantification of fungal growth by polymerase chain reaction (PCR) and photometric assay
  • in conventional reverse transcriptase (RT)-PCR
  • in simple sequence repeats (SSR) genotyping
  • as a component of PCR mix for amplification of genomic and mitochondrial DNA
  • in direct tetra-primer amplification refractory mutation system (T-ARMS) PCR to amplify dried whole blood samples

생화학적/생리학적 작용

Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.

특징 및 장점

  • Low per unit cost of Taq

포장

Taq DNA Polymerase with 10× reaction buffer containing MgCl2
Taq DNA polymerase comes with the choice of an optimized 10× reaction buffer including MgCl2 (D1806) or a 10× reaction buffer without MgCl2 plus a separate tube of MgCl2 for titration (D4545). The latter option may be necessary to determine optimal conditions for amplification.

기타 정보

One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.
Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. It has both 5′→3′ polymerase and exonuclease activity.

법적 정보

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Storage Class Code

12 - Non Combustible Liquids

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
SLCL7739
SLCJ5971
SLCK0052
SLCJ6870
SLCJ8540

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문서

Introduction & Historical Timelines

Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.

프로토콜

표준 PCR 프로토콜

표준 PCR 프로토콜 단계를 알아보고 시약 목록 또는 사이클링 매개변수를 검토하세요. 일상적인 DNA 증폭을 위한 이 방법은 표준 Taq DNA 중합효소를 사용합니다.

dNTP Hot Start PCR Protocol

Hot Start dNTPs block DNA polymerase until heat activation, enhancing PCR specificity.

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Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

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