B1911
BCIP®/NBT
alkaline phosphatase substrate, chromogenic, liquid
동의어(들):
5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride solution, BCIP/NBT Alkaline Phosphatase Substrate solution
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크기 선택
보기 변경
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352204
NACRES:
NA.83
제품 이름
BCIP®/NBT Liquid Substrate System, ready to use solution
Quality Level
form
liquid
storage temp.
2-8°C
Application
A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.
BCIP®/NBT Liquid Substrate System has been used:
- for staining alkaline phosphatase(ALP) in bone marrow stromal cells
- as a substrate in western blot detection of matrix metalloproteinase-2 specific antibody
- as a substrate for streptavidin-alkaline phosphatase in the enzyme-linked immunospot (ELISpot) assays
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
It is recommended to store the product between 4 - 8 °C. Do not freeze. The product may be stored at room temperature for up to one year. If a precipitate forms, the solution can be gently swirled or filtered.
저장 등급
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
프로토콜
BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.
문서
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
관련 콘텐츠
Commensal microbes induce serum IgA responses that protect against polymicrobial sepsis
Wilmore JR, et al.
Cell host & microbe, 23(3), 302-311 (2018)
Nana H Overgaard et al.
Vaccine, 35(42), 5629-5636 (2017-09-10)
The relationship between the antigen dose and the quality of an immune response generated upon immunization is poorly understood. However, findings show that the immune system is indeed influenced by the antigen dose; hence underlining the importance of correctly determining
Epigenetic regulation of Runx2 transcription and osteoblast differentiation by nicotinamide phosphoribosyltransferase
Ling M, et al.
Cell & Bioscience, 7(1), 27-27 (2017)
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| B1911-100ML | 04061838251626 |