A9647

Bovine Serum Albumin

Name: Bovine Serum Albumin
Brand: SIGMA

heat shock fraction, pH 7, ≥98%

동의어(들):

Albumin bovine serum, BSA, Bovine albumin

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

CAS 번호:

9048-46-8

EC Number:

232-936-2

MDL number:

MFCD00130384

UNSPSC 코드:

12352202

NACRES:

NA.27

주요 문서

모든 문서 보기

기술 서비스

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도움 문의

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도움 문의

생물학적 소스

bovine

Quality Level

300

분석

≥98%

양식

lyophilized powder

분자량

~66 kDa

정제법

heat shock fractionation

기원

USA origin

기술

immunocytochemistry: suitable

불순물

≤5.0% Loss on drying

손실

≤5%

pH

solubility

water: soluble (40 mg/ml)

UniProt 수납 번호

P02769

외래 활성

BT virus, none detected
VSV virus, none detected

저장 온도

2-8°C

유전자 정보

bovine ... ALB(280717)

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일반 설명

Bovine serum albumin is a protein whose key biological function is to regulate the colloidal osmotic pressure of blood. BSA is widely used in various studies and applications. Made up of 583 amino acids, BSA is a water-soluble protein with a calculated molecular weight of 66,430 Da. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.

애플리케이션

Bovine Serum Albumin is used for many applications, including the following applications:

BSA has been used in cell culture, e.g. as a supplement in media prepared for culturing of oligodendrocytes.
BSA has been used for the dilution of primary antibody solution in immunohistochemistry procedures.
BSA has been used as a standard in the Bradford method of protein quantification.
BSA has been used in blocking and permeabilization buffer for immunofluorescence studies.

생화학적/생리학적 작용

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

제조 메모

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

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시험 성적서(COA)

Lot/Batch Number

0000279981

MJR0000002

SLCL4075

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모든 관련된 서류 보기

문서

Fast Analysis of Monoclonal Antibodies Using Silica Monoliths Designed for Bioanalysis

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Albumin in Cell Culture

Explore serum albumin's role in serum-free cell culture systems for biomanufacturing.

Colorimetric Detection of Western blot using 4-Chloro-1-Naphthol Solution Protocol

This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.

프로토콜

Enzymatic Assay of Alcohol Dehydrogenase (EC 1.1.1.1)

To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Bovine Serum Albumin

heat shock fraction, pH 7, ≥98%

크기 선택

제품정보 (DICE 배송 시 비용 별도)

주요 문서

속성

생물학적 소스

Quality Level

분석

양식

분자량

정제법

기원

기술

불순물

손실

pH

solubility

UniProt 수납 번호

외래 활성

저장 온도

유전자 정보

관련 카테고리

설명

일반 설명

애플리케이션

생화학적/생리학적 작용

제조 메모

안전성 정보

Storage Class Code

WGK

Flash Point (°F)

Flash Point (°C)

개인 보호 장비

문서

시험 성적서(COA)

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