A4464
Enhanced Avian Reverse Transcriptase [eAMV™ RT]
For reverse transcription at higher temperatures & rare mRNAs
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크기 선택
보기 변경
제품정보 (DICE 배송 시 비용 별도)
Concentration:
20 units/μL
Quality Level
form
crystalline powder
usage
sufficient for 50 reactions
feature
dNTPs included: no, hotstart: no
concentration
20 units/μL
technique(s)
RT-PCR: suitable, cDNA synthesis: suitable
color
colorless
input
purified RNA
shipped in
wet ice
storage temp.
−20°C
General description
eAMV™ Reverse Transcriptase is an enhanced form of Avian Myeloblastosis Virus (AMV) RT that synthesizes a DNA strand complementary to RNA, DNA, or an RNA:DNA hybrid. This exceptionally robust AMV RT has greater thermostability than standard AMV or Moloney murine leukemia virus(M-MLV) reverse transcriptase. eAMV™ RT is an ideal enzyme for producing high-quality full-length cDNA from total RNA or poly(A)+ RNA and is also efficient at transcribing long targets.
Application
Enhanced Avian (eAMV) Reverse Transcriptase is a highly purified, avian myeloblastosis virus reverse transcriptase (AMV-RT) that offers superior performance in comparison to standard AMV-RT or standard Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT).
This exceptionally robust AMV-RT has an enhanced ability to detect low abundance messages, transcribe through difficult secondary structure at elevated temperatures (up to 65 °C), and transcribe mRNA templates up to 14.1 kb.
This exceptionally robust AMV-RT has an enhanced ability to detect low abundance messages, transcribe through difficult secondary structure at elevated temperatures (up to 65 °C), and transcribe mRNA templates up to 14.1 kb.
Enhanced Avian Reverse Transcriptase (eAMV RT) is used to transcribe RNA into DNA, and facilitates efficient mRNA template driven sysnthesis of cDNAs. This is due to the abillity of this enhanced AMV-RT to transcribe large mRNA templates, to transcribe through difficult secondary structures, and to detect low abundance mRNAs by RT-PCR.
Enhanced Avian Reverse Transcriptase [eAMV™ RT] has been used for reverse transcription of total RNA to synthesize cDNA during quantitative reverse transcription PCR (RT-qPCR) analysis.
Biochem/physiol Actions
Reverse transcriptase catalyzes RNA template incorporation of dNTPs into complimantary DNA through phosphodiester bond formation.
Features and Benefits
- Greater sensitivity for low abundance mRNA
- Unsurpassed transcription through difficult secondary structures at elevated temperatures (up to 65°C)
- Efficient generation of full-length cDNA, up to 14.1 kb
- Produces first strand cDNA ready for PCR amplification
Packaging
Provided with a vial of 10× reaction buffer.
Other Notes
One unit incorporates one nanomole of TMP into TCA precipitable material in 10 min using polyadenylic acid as template and oligo(dT)12-18 as a primer.
Legal Information
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
eAMV is a trademark of Sigma-Aldrich Co. LLC
저장 등급
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
문서
Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.
관련 콘텐츠
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 5-5 (1989)
C Tosh et al.
Acta virologica, 41(3), 153-155 (1997-06-01)
A method of reverse transcription (RT) and polymerase chain reaction (PCR) amplification of 1D (VP1) gene of foot-and-mouth disease (FMD) virus using one reaction mixture containing both avian myeloblastosis virus (AMV) reverse transcriptase (RTase) and Tfl DNA polymerase is described.
Emily Schifano et al.
Virulence, 10(1), 1013-1025 (2019-11-28)
Calcium signaling can elicit different pathways involved in an extreme variety of biological processes. Calcium levels must be tightly regulated in a spatial and temporal manner in order to be efficiently and properly utilized in the host physiology. The Ca2+-ATPase
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| A4464-1KU | 04061833374504 |