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Merck

SEQXE

SeqPlex DNA Amplification Kit

For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing.

동의어(들):

SeqPlex Enhanced DNA Amplification Kit, WGA kit, whole genome amplification kit

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제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.55
UNSPSC Code:
41121800
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Quality Level

technique(s)

whole genome amplification: suitable

dilution

(WGA)

input

purified DNA

shipped in

wet ice

storage temp.

−20°C

General description

SeqPlex Enhanced DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA. The yields from chromatin immunoprecipitation (ChIP) or formalin-fixed paraffin-embedded tissue samples (FFPE) are often less than required for successful next generation sequencing library preparation. The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented DNA samples for input into a NGS workflow. This kit is an extension of the WGA product line and has been developed to integrate into the Illumina®, SOLiD, or 454 sequencing workflows.

Application

SeqPlex DNA Amplification Kit has been used for whole genome amplification.

Features and Benefits

  • Random priming technology amplifies fragmented DNA such as ChIP or FFPE
  • Facilitates sequencing from as little as 100 pg of ChIP DNA
  • Enhanced primers for complete genome coverage, minimal sequence bias, primer removal, and amplicon size ideal for next gen sequencing
  • Compatible with Illumina®, SOLiD, or 454 library prep for next generation sequencing

Other Notes

SEQXE-500RXN is manufactured on-demand. Contact technical services at techserv@sial.com for more information.

Legal Information

Illumina is a registered trademark of Illumina, Inc.
SOLiD is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SeqPlex is a trademark of Sigma-Aldrich Co. LLC


키트 구성품 역시 별도로 이용 가능함

제품 번호
설명
SDS & 가격

  • Water, Nuclease-Free Water, for Molecular Biology
    SDS

  • Library Preparation Buffer
    SDS

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

저장 등급

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3



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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문


프로토콜

The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format

SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.


Dimiter Kunnev et al.
Journal of biological methods, 2(4) (2015-01-01)
Nascent strand capture and release (NSCR) is a method for isolation of short nascent strands to identify origins of DNA replication. The protocol provided involves isolation of total DNA, denaturation, size fractionation on a sucrose gradient, 5'-biotinylation of the appropriate
Joshua A Udall et al.
Frontiers in plant science, 10, 1541-1541 (2019-12-13)
One of the extraordinary aspects of plant genome evolution is variation in chromosome number, particularly that among closely related species. This is exemplified by the cotton genus (Gossypium) and its relatives, where most species and genera have a base chromosome
Takuya Hayakawa et al.
Cytogenetic and genome research, 161(8-9), 437-444 (2021-11-25)
E/L Repli-seq is a powerful tool for detecting cell type-specific replication landscapes in mammalian cells, but its potential to monitor DNA replication under replication stress awaits better understanding. Here, we used E/L Repli-seq to examine the temporal order of DNA



국제 무역 품목 번호

SKUGTIN
SEQXE-10RXN04061837018138
SEQXE-500RXN04061837018145
SEQXE-50RXN04061837018176