S9811
S-Gal®
reagent for selection of recombinant bacterial clones
동의어(들):
3,4-Cyclohexenoesculetin β-D-galactopyranoside
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
크기 선택
보기 변경
제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
C19H22O9
CAS 번호:
Molecular Weight:
394.37
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.85
grade
Molecular Biology
Quality Level
sterility
non-sterile
assay
≥95% (HPLC)
form
powder
solubility
DMF: 50 mg/mL
suitability
suitable for β-galactosidase test
storage temp.
room temp
SMILES string
OC[C@H]1O[C@@H](Oc2cc3OC(=O)C4=C(CCCC4)c3cc2O)[C@H](O)[C@@H](O)[C@H]1O
InChI
1S/C19H22O9/c20-7-14-15(22)16(23)17(24)19(28-14)27-13-6-12-10(5-11(13)21)8-3-1-2-4-9(8)18(25)26-12/h5-6,14-17,19-24H,1-4,7H2/t14-,15+,16+,17-,19-/m1/s1
InChI key
HDJJDRXZHJRVGA-DIKXUDHVSA-N
General description
S-Gal® is an autoclavable, chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. S-Gal® is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Application
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. S-Gal® is autoclavable and can be added to bacterial broth containing agar prior to autoclaving.
S-Gal is a patented autoclavable chromogenic substrate for β-galactosidase that is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Biochem/physiol Actions
When S-Gal® is cleaved by β-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal® and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies.
Features and Benefits
- More intense color contrast than X-gal
- Excellent for use in automated colony counters
- Autoclavable for easiest use
- No need to make stock solutions
When S-Gal is cleaved by β-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies. S-Gal is autoclavable and can be added to your medium of choice prior to autoclaving. S-Gal is not light sensitive and does not require any protection from light sources.
Preparation Note
Stock solutions of S-Gal® can be made by dissolving 50mg/ml in dimethyl formamide (DMF) and storing at -20C. Add S-Gal® (300 mg/L from stock solution ) and Ferric Ammonium Citrate (500mg/L) to agar media prior to autoclaving.
Other Notes
Autoclavable, microwavable growth medium complete with S-Gal and IPTG.
The ferric or Fe3+ ion is required for color development and must be added to any S-Gal®
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.
Legal Information
S-GAL is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
프로토콜
Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.
문서
General protocols for growth of competent cells and their transformation (uptake of DNA).
S Fitzpatrick et al.
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 9(2), 206-209 (2008-12-09)
Rhodnius prolixus is the main vector of Chagas disease in Venezuela, where it is found colonising rural housing consisting of unplastered adobe walls with palm and/or metal roofs. Vector control failure in Venezuela may be due to the invasion of
Development and characterization of ten polymorphic microsatellites isolated from the scallop Argopecten purpuratus.
C J Mandiola-Quililongo et al.
Journal of genetics, 91(1), e12-e14 (2012-05-04)
Christopher Voigt
Synthetic Biology: Methods for part/device characterization and chassis engineering (2011)
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| S9811-1G | 04061836962166 |
| S9811-5G | 04061832745244 |
| S9811-100MG | 04061838355690 |