B6650
X-GlcA
reagent for selection of recombinant bacterial clones
동의어(들):
5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, X-GlcA, X-glucuronide CHA salt
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크기 선택
보기 변경
제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
C14H13BrClNO7 · C6H13N
CAS 번호:
Molecular Weight:
521.79
NACRES:
NA.85
PubChem Substance ID:
UNSPSC Code:
12352200
MDL number:
grade
Molecular Biology
Quality Level
sterility
non-sterile
assay
≥98% (TLC)
form
powder
technique(s)
nucleic acid detection: suitable
solubility
DMF: soluble
suitability
suitable for β-galactosidase test
storage temp.
−20°C
SMILES string
NC1CCCCC1.O[C@@H]2[C@@H](O)[C@@H](O[C@@H]([C@H]2O)C(O)=O)Oc3c[nH]c4ccc(Br)c(Cl)c34
InChI
1S/C14H13BrClNO7.C6H13N/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;7-6-4-2-1-3-5-6/h1-3,9-12,14,17-20H,(H,21,22);6H,1-5,7H2/t9-,10-,11+,12-,14+;/m0./s1
InChI key
JXCKZXHCJOVIAV-CYRSAHDMSA-N
General description
X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Application
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.
X-GlcA has been used in histochemical staining of root sections for light microscopic observation.
X-GlcA has been used in histochemical staining of root sections for light microscopic observation.
Chromogenic substrate for β-glucuronidase (GUS) gene detection.
Biochem/physiol Actions
When X-GlcA is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of X-GlcA turn an intense blue color, allowing for easy differentiation between lac+ and lac- colonies.
문서
Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.
Stéphane Compant et al.
Applied and environmental microbiology, 71(4), 1685-1693 (2005-04-07)
Patterns of colonization of Vitis vinifera L. cv. Chardonnay plantlets by a plant growth-promoting bacterium, Burkholderia sp. strain PsJN, were studied under gnotobiotic conditions. Wild-type strain PsJN and genetically engineered derivatives of this strain tagged with gfp (PsJN::gfp2x) or gusA
Differential colonization of tomato roots by nonpathogenic and pathogenic Fusarium oxysporum strains may influence Fusarium wilt control
Bao J R and Lazarovits G
Phytopathology, 91(5), 449-456 (2001)
Li Liu et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 21(9), 2014-2019 (2007-03-14)
Gene therapy shows promise for treating prostate cancer and has been evaluated in several clinical trials. A major challenge that remains is to establish a method for verifying transgene activity in situ. The lacZ gene encoding beta-galactosidase historically has been
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| B6650-100MG | 04061833435373 |
| B6650-10MG | 04061833435441 |
| B6650-25MG | 04061833435496 |