General description
The PhasePrep BAC DNA Kit offers a scalable and cost-effective method for isolating large-molecular weight plasmids such as Bacterial Artificial Chromosomes (BAC) from recombinant E. coli cultures. The same kit can be used for preparations of four different sizes; sufficient reagents are provided for 300 micro, 180 mini, 30 midi, or 15 maxi preparations. Up to 2,12, 50, or 100 mg of BAC DNA can be recovered from 5, 40, 250, or 500 mL of overnight recombinant E. coli culture, respectively. The purified BAC DNA contains very low levels of endotoxin (≤10 EU/μg DNA).
Recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure. Nucleic acid is precipitated from the cleared lysate; residual RNA is removed by a short digestion at elevated temperature with an RNase cocktail. Endotoxins and other impurities are removed by simple temperature-mediated extraction and phase separation. Finally the BAC DNA is selectively precipitated from solution.
The recovered BAC DNA is predominantly in its super-coiled form, free of RNA contamination, and ready for immediate use in downstream applications, such as sequencing, restriction digestion, cloning, and PCR.
Recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure. Nucleic acid is precipitated from the cleared lysate; residual RNA is removed by a short digestion at elevated temperature with an RNase cocktail. Endotoxins and other impurities are removed by simple temperature-mediated extraction and phase separation. Finally the BAC DNA is selectively precipitated from solution.
The recovered BAC DNA is predominantly in its super-coiled form, free of RNA contamination, and ready for immediate use in downstream applications, such as sequencing, restriction digestion, cloning, and PCR.
The PhasePrep™ BAC DNA Kit offers a scalable and cost-effective method for isolating large-molecular weight plasmids such as Bacterial Artificial Chromosomes (BAC) from recombinant E. coli cultures. The same kit can be used for preparations of four different sizes; sufficient reagents are provided for 300 micro, 180 mini, 30 midi, or 15 maxi preparations. Up to 2,12, 50, or 100 mg of BAC DNA can be recovered from 5, 40, 250, or 500 ml of overnight recombinant E. coli culture, respectively. The purified BAC DNA contains very low levels of endotoxin (£10 EU/mg DNA).
Recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure. Nucleic acid is precipitated from the cleared lysate; residual RNA is removed by a short digestion at elevated temperature with an RNase cocktail. Endotoxins and other impurities are removed by simple temperature-mediated extraction and phase separation. Finally the BAC DNA is selectively precipitated from solution.
Recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure. Nucleic acid is precipitated from the cleared lysate; residual RNA is removed by a short digestion at elevated temperature with an RNase cocktail. Endotoxins and other impurities are removed by simple temperature-mediated extraction and phase separation. Finally the BAC DNA is selectively precipitated from solution.
Application
PhasePrep™ BAC DNA Kit has been used to isolate bacterial artificial chromosomes.
The recovered BAC DNA is predominantly in its super-coiled form, free of RNA contamination, and ready for immediate use in downstream applications, such as sequencing, restriction digestion, cloning, and PCR.
Features and Benefits
- Typical DNA yields of 2-100 μg from 5-500 mL of overnight cultures
- No phenol/chloroform extraction required
- Allows possible micro to maxi preps with the same kit
- No long waits for drip columns
- Typical DNA yields of 2-100 μg from 5-500 mL of overnight cultures
- Allows possible micro to maxi preps with the same kit
- No long waits for drip columns
Other Notes
For additional information, please see www.sigma-aldrich.com/genelutehp.
The PhasePrep™ BAC DNA Kit offers a highly scalable, rapid, cost-effective method for isolating high molecular weight plasmids such as Bacterial Artificial Chromosomes (BACs) from recombinant E. coli cultures.
Legal Information
PhasePrep is a trademark of Sigma-Aldrich Co. LLC
키트 구성품 역시 별도로 이용 가능함
제품 번호
설명
SDS & 가격
- Sodium acetate buffer solution, pH 7.0±0.05 (25 °C), BioXtra, Molecular Biology, 3 M, non-sterile; 0.2 μm filtered
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
저장 등급
10 - Combustible liquids
Comparative structural analysis of Bru1 region homeologs in Saccharum spontaneum and S. officinarum
Jisen Zhang
BMC Genomics, 17 (2016)
Evolution and expression of the fructokinase gene family in Saccharum
Yihong Chen
BMC Genomics, 18, 197-197 (2017)
Structure, phylogeny, allelic haplotypes and expression of sucrose transporter gene families in Saccharum
Qing Zhang
BMC Genomics, 17 (2016)
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| NA0100-1KT | 04061834118985 |