1.0% Agarose in Tris-Acetate EDTA Buffer

1% Agarose in Tris Acetate EDTA (TAE buffer, pH 8.3) is a powder blend that readily dissolves in boiling DI or ultrapure water to quickly make an agarose gel for electrophoresis. Ideal for separatation of DNA or RNA fragments that range from 400 – 8,000 bp. Gels with TAE buffer are typically used for electrophoresis of larger nucleic acid fragments.

1% Agarose in 1X TAE buffer may be used to make a gel for:

• Native RNA gel electrophoresis
• DNA gel electrophoresis

Foil pouches

Contents of one pouch, when dissolved in 250 mL of ultrapure water, will yield a 1× solution containing 40 mM Tris acetate, 1 mM EDTA, and 1.0% (w/v) agarose. Contents tested to be DNAse, RNAse, and Nickase free. Prepare in a 1L flask. Combine pHast pack contents with 250 mL ultrapure water and mix well. Microwave on high for ~1min to boil and dissolve the agarose. Use a heat resistant glove to remove the flask every 10 - 15s to mix gently by swirling the liquid in a circle at the bottom of the flask. Repeat until the agarose is fully dissolved. Cool the flask slightly with cold water while swirling every 10 – 15s, add the stain and pour the gel. Allow agarose gel to fully cool and solidify before removing combs.

Prepares 250mL for casting several standard-size gels or one large agarose gel. A small electrophoresis apparatus maybe use 30 - 50mL, medium 100mL, and large rigs 250mL. Add nucleic acid stain before pouring the gel and for optimal results pair with pHast pack 1X TAE, pH 8.3 running buffer.

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

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