P2308

Proteinase K from Tritirachium album

Name: Proteinase K from <I>Tritirachium album</I>
Brand: SIAL

lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg protein

동의어(들):

Endopeptidase K

로그인조직 및 계약 가격 보기

크기 선택

제품정보 (DICE 배송 시 비용 별도)

CAS 번호:

39450-01-6

EC 번호:

3.4.21.64 (BRENDA, IUBMB)

EC Number:

254-457-8

MDL number:

MFCD00132129

UNSPSC 코드:

12352204

eCl@ss:

32160410

NACRES:

NA.54

주요 문서

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도움 문의

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도움 문의

Grade

Molecular Biology

Quality Level

400

제품 라인

BioUltra

양식

lyophilized powder

특이 활성도

≥30 units/mg protein

분자량

28.93 kDa

구성

Protein, ≥90% biuret

불순물

≤1 ppm DNA

외래 활성

DNAse, Nickase and RNAse, none detected

배송 상태

wet ice

저장 온도

−20°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

애플리케이션

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

생화학적/생리학적 작용

Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp³⁹-His⁶⁹-Ser²²⁴). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

기타 정보

One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

픽토그램

GHS08,GHS07

신호어

Danger

유해 및 위험 성명서

H315,H319,H334,H335

예방조치 성명서

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

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COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Microfluidic PCR Amplification and MiSeq Amplicon Sequencing Techniques for High-Throughput Detection and Genotyping of Human Pathogenic RNA Viruses in Human Feces, Sewage, and Oysters.

Mamoru Oshiki et al.

Frontiers in microbiology, 9, 830-830 (2018-05-15)

Detection and genotyping of pathogenic RNA viruses in human and environmental samples are useful for monitoring the circulation and prevalence of these pathogens, whereas a conventional PCR assay followed by Sanger sequencing is time-consuming and laborious. The present study aimed

m6A modification suppresses ocular melanoma through modulating HINT2 mRNA translation.

Ruobing Jia et al.

Molecular cancer, 18(1), 161-161 (2019-11-15)

Dynamic N6-methyladenosine (m6A) RNA modification generated and erased by N6-methyltransferases and demethylases regulates gene expression, alternative splicing and cell fate. Ocular melanoma, comprising uveal melanoma (UM) and conjunctival melanoma (CM), is the most common primary eye tumor in adults and

Coordination of Hepatitis C Virus Assembly by Distinct Regulatory Regions in Nonstructural Protein 5A.

Margarita Zayas et al.

PLoS pathogens, 12(1), e1005376-e1005376 (2016-01-05)

Hepatitis C virus (HCV) nonstructural protein (NS)5A is a RNA-binding protein composed of a N-terminal membrane anchor, a structured domain I (DI) and two intrinsically disordered domains (DII and DIII) interacting with viral and cellular proteins. While DI and DII

Effect of wet storage conditions on potato tuber transcriptome, phytohormones and growth.

Bahram Peivastegan et al.

BMC plant biology, 19(1), 262-262 (2019-06-19)

Stored potato (Solanum tuberosum L.) tubers are sensitive to wet conditions that can cause rotting in long-term storage. To study the effect of water on the tuber surface during storage, microarray analysis, RNA-Seq profiling, qRT-PCR and phytohormone measurements were performed

Kidney decellularized extracellular matrix hydrogels: Rheological characterization and human glomerular endothelial cell response to encapsulation.

Jimmy Su et al.

Journal of biomedical materials research. Part A, 106(9), 2448-2462 (2018-04-18)

Hydrogels, highly-hydrated crosslinked polymer networks, closely mimic the microenvironment of native extracellular matrix (ECM) and thus present as ideal platforms for three-dimensional cell culture. Hydrogels derived from tissue- and organ-specific decellularized ECM (dECM) may retain bioactive signaling cues from the

모든 관련된 서류 보기

문서

Best Product & Supplier for Proteinase K

Balancing Proteinase K cost with quality and technical support ensures optimal enzyme selection for diverse applications.

Guide on How to Use Proteinase K in Different Procedures

Guidelines on use of proteinase K, an enzyme commonly used to degrade proteins, and protect DNA and RNA from degradation in samples.

How Proteinase K Can Be Used in Blood DNA Extraction Applications

In blood DNA extraction, Proteinase K, an enzyme commonly used to degrade proteins, can help break down the cellular and nuclear membranes, releasing DNA from the cells that protect it from degradation and increase purity/yield making it more suitable for various molecular biology techniques.

Proteinase K in Tissue Samples

Pro K aids in disrupting cell membranes for DNA release, crucial for downstream molecular biology techniques.

모두 보기

프로토콜

GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

GenElute™ Blood Genomic DNA Kit Protocol

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

GenElute™ Mammalian Genomic DNA Miniprep Kit Protocol

The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.

Proteinase K Enzymatic Assay

Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.

Proteinase K from Tritirachium album

lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg protein

크기 선택

제품정보 (DICE 배송 시 비용 별도)

주요 문서

속성

Grade

Quality Level

제품 라인

양식

특이 활성도

분자량

구성

불순물

외래 활성

배송 상태

저장 온도

관련 카테고리

설명

애플리케이션

생화학적/생리학적 작용

기타 정보

안전성 정보

픽토그램

신호어

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

표적 기관

Storage Class Code

WGK

Flash Point (°F)

Flash Point (°C)

개인 보호 장비

문서

시험 성적서(COA)

이 제품을 이미 가지고 계십니까?

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