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NIST8671

NISTmAb, Humanized IgG1k Monoclonal Antibody

NIST® SRM® 8671

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제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.41
UNSPSC Code:
41116107
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도움 문의

antibody product type

primary antibodies

grade

certified reference material

form

liquid

manufacturer/tradename

NIST®

technique(s)

UV/Vis spectroscopy: suitable, liquid chromatography (LC): suitable, mass spectrometry (MS): suitable

format

matrix material

General description

NISTmAb, Humanized IgG1k Monoclonal Antibody is a recombinant antibody expressed in murine suspension culture. It is a homodimer with two identical light chains and two identical heavy chains linked through inter- and intra- chain disulfide bonds with a molecular weight of 150 kDa. The protein possesses a low abundance of post-translational modifications including methionine oxidation, deamidation, and glycation. This humanized monoclonal antibody also has N-terminal pyroglutamination, C-terminal lysine clipping, and glycosylation of the heavy chains.

A unit of RM 8671 consists of one internal-threaded polypropylene cryovial containing 800 µL of 10 mg/mL NIST IgG1κ monoclonal antibody (NISTmAb) in 12.5 mmol/L L-histidine, 12.5 mmol/L L-histidine HCl (pH 6.0).

SRM 8671_Cert

SRM 8671_SDS

Application

NISTmAb, Humanized IgG1k Monoclonal Antibody has been used as a standard for testing de novo sequencing algorithms on bottom-up mass spectrometry.This reference material (RM) is primarily intended for evaluating the performance of methods for determining physiochemical and biophysical attributes of monoclonal antibodies. It also provides a representative test molecule for developing novel technology in therapeutic protein characterization. Other potential uses include:
  • system suitability tests
  • establishing method or instrument performance and variability
  • comparing changing analytical test methods
  • assisting in method qualification

Analysis Note

NIST8671 is intended for research use. It is not intended for animal or human consumption, clinical testing or therapeutic use.

Other Notes

  • RM 8671 is intended for research use.
  • Details on expiration, storage, safety, usage, and source are provided in the NIST certificate. 
  • Information on biomaterials, disposal, and transport is available in the SDS.

Legal Information

NIST is a registered trademark of National Institute of Standards and Technology
SRM is a registered trademark of National Institute of Standards and Technology

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저장 등급

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

Middle-Up Antibody Analysis by RP Chromatography

Compare columns in resolving medium-sized antibody fragments after digestion with DTT or IdeS using Reversed-Phase Chromatography for analysis.

Charge Variant Analysis (CVA) with Strong Cation Exchange

Learn about the separation of several charge variants of two monoclonal antibodies, NISTmAb and Infliximab, using a polymeric cation exchange column and MS-compatible mobile phases.

An Optimized Protocol for Peptide Mapping of Therapeutic Monoclonal Antibodies with Minimum Deamidation and Oxidation Artifacts

An optimized peptide mapping protocol using NISTmAb as a model monoclonal antibody, shorter incubation times, and improved digestion buffer to demonstrate minimal artificial asparagine deamidation and methionine oxidation.

모든 기사 보기

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Intact Mass Analysis of Monoclonal Antibodies

Discover our wide variety of products for intact mass analysis of monoclonal antibodies, including size-exclusion columns (SEC), ion exchange columns, reverse-phase columns, HPLC buffers, MALDI matrices and standards, high-purity solvents, reagents, tools for protein sample preparation, and certified reference materials.

Ioannis Karageorgos et al.
Biologicals : journal of the International Association of Biological Standardization, 50, 27-34 (2017-10-03)
Monoclonal antibody pharmaceuticals are the fastest-growing class of therapeutics, with a wide range of clinical applications. To assure their safety, these protein drugs must demonstrate highly consistent purity and stability. Key to these objectives is higher order structure measurements validated
Automated Antibody De Novo Sequencing and Its Utility in Biopharmaceutical Discovery.
Sen K I, et al.
Journal of the American Society For Mass Spectrometry, 28(5), 803-810 (2017)

국제 무역 품목 번호

SKUGTIN
NIST867104061838660374