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Merck

N6876

Nitrotetrazolium Blue chloride

≥90.0% (HPLC)

동의어(들):

2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT

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제품정보 (DICE 배송 시 비용 별도)

Linear Formula:
C40H30N10O6 · 2Cl
CAS 번호:
Molecular Weight:
817.64
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
4115923
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Quality Level

assay

≥90.0% (HPLC)

form

powder

impurities

≤10% solvent

solubility

H2O: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8

InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

General description

Substrate for dehydrogenases and other oxidases.

Application

  • Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.
  • It has been used as a substrate for western blot detection.
  • It has been used for the staining of histological tissue sections.

Biochem/physiol Actions

Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis


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pictograms

Health hazardExclamation mark

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 2 - STOT SE 3

target_organs

Eyes,Central nervous system, Respiratory system

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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문서 라이브러리 방문


문서

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.


Measurement of reactive oxygen intermediate production in haemocytes of the penaeid shrimp, Penaeus ?annamei.
Knaap V et al.
Aquaculture (Amsterdam, Netherlands), 191, 89-107 (2000)
Expression of the endothelial lipase gene in murine embryos and reproductive organs.
Lindegaard ML et al.
Journal of Lipid Research, 46, 439-439 (2005)
Drought- and salt-tolerant plants result from overexpression of the AVP1 H+-pump.
Gaxiola RA et al.
Proceedings of the National Academy of Sciences of the USA, 98, 11444-11444 (2001)



국제 무역 품목 번호

SKUGTIN
N6876-10X10MG04061834117728
N6876-100MG04061835573585
N6876-1G04061835573592
N6876-250MG04061835573608
N6876-500MG04061835573615
N6876-50MG04061834117735
N6876-5G04061835573622