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Merck

A0576

Agarose

Low EEO

동의어(들):

3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose LE

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제품정보 (DICE 배송 시 비용 별도)

CAS 번호:
UNSPSC Code:
41105317
PubChem Substance ID:
EC Number:
232-731-8
NACRES:
NA.21
MDL number:
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Quality Level

type

Type I-B

form

powder

technique(s)

electrophoresis: suitable

impurities

≤7% water

ash

≤0.25%

turbidity

≤4 NTU (1.5% gel)

EEO

≤0.12

mp

86.5-89.5  °C

transition temp

gel point 36 °C ±1.5 °C (1.5% gel)

gel strength

≥1800 g/cm2 (1% gel), ≥3200 g/cm2 (1.5% gel)

anion traces

sulfate (SO42-): ≤0.12%

SMILES string

O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

General description

Agarose, a natural polymer is obtained from sea algae. This neutral linear polysaccharide is made up of d-galactose and 3,6-anhydro-l-galactose. These are connected alternatively with the help of glycosidic bonds. Agarose has high significance in gel electrophoresis and gel chromatography.

Application

Agarose has been used:
  • to form the photosynthetic slab
  • in comparative study, to compare it with native agar, and agarose of Gracilaria dura
  • to estimate the amino acid concentrations in agarose and agar samples
  • as immobilisation substrates for enzyme immobilisation
  • for separating high molecular weight nucleic acids at low gel concentrations

Biochem/physiol Actions

Agarose is used as bioink due because of its characteristic properties such as, biocompatibility, mechanical strength and gelling ability at low temperature.

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Other Notes

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저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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3D Bioprinting for Reconstructive Surgery, 277-304 (2018)
Preparation, characterization and benchmarking of agarose from Gracilaria dura of Indian waters
Meena R, et al.
Carbohydrate Polymers, 69(1), 179-188 (2007)
Determination of amino acids in diverse polymeric matrices using HPLC, with emphasis on agars and agaroses
Palace GP, et al.
Biochimica et Biophysica Acta, 1472(3), 509-518 (1999)



국제 무역 품목 번호

SKUGTIN
A0576-100G04061833334119
A0576-25G04061833608098