Quality Level
type
Type I-B
form
powder
technique(s)
electrophoresis: suitable
impurities
≤7% water
ash
≤0.25%
turbidity
≤4 NTU (1.5% gel)
EEO
≤0.12
mp
86.5-89.5 °C
transition temp
gel point 36 °C ±1.5 °C (1.5% gel)
gel strength
≥1800 g/cm2 (1% gel), ≥3200 g/cm2 (1.5% gel)
anion traces
sulfate (SO42-): ≤0.12%
SMILES string
O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O
InChI
1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key
MJQHZNBUODTQTK-WKGBVCLCSA-N
General description
Agarose, a natural polymer is obtained from sea algae. This neutral linear polysaccharide is made up of d-galactose and 3,6-anhydro-l-galactose. These are connected alternatively with the help of glycosidic bonds. Agarose has high significance in gel electrophoresis and gel chromatography.
Application
Agarose has been used:
- to form the photosynthetic slab
- in comparative study, to compare it with native agar, and agarose of Gracilaria dura
- to estimate the amino acid concentrations in agarose and agar samples
- as immobilisation substrates for enzyme immobilisation
- for separating high molecular weight nucleic acids at low gel concentrations
Biochem/physiol Actions
Agarose is used as bioink due because of its characteristic properties such as, biocompatibility, mechanical strength and gelling ability at low temperature.
Analysis Note
The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
3D bioprinting cartilage
3D Bioprinting for Reconstructive Surgery, 277-304 (2018)
Preparation, characterization and benchmarking of agarose from Gracilaria dura of Indian waters
Meena R, et al.
Carbohydrate Polymers, 69(1), 179-188 (2007)
Determination of amino acids in diverse polymeric matrices using HPLC, with emphasis on agars and agaroses
Palace GP, et al.
Biochimica et Biophysica Acta, 1472(3), 509-518 (1999)
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| A0576-100G | 04061833334119 |
| A0576-25G | 04061833608098 |