UFC5030
Amicon® Ultra Centrifugal Filter, 30 kDa MWCO
sample volume 0.5 mL, regenerated cellulose membrane, MWCO 30 kDa
동의어(들):
Centrifugal concentrator, 30 kDa MWCO, 0.5 mL sample volume
크기 선택
제품정보 (DICE 배송 시 비용 별도)
Quality Level
material
polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg, pkg of 500 pkg, pkg of 8 pkg, pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
0.5 mL sample volume
technique(s)
protein extraction: suitable, protein purification: suitable
L
4.99 cm (1.96 in.)
diam.
10.8 mm
filtration area
1 cm2
volume
0.5 mL
working volume
≤500 μL , 0.5 mL
pore size
30 kDa MWCO
greener alternative category
shipped in
ambient
General description
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
Application
- Ultrafiltration with 30,000 Dalton molecular weight cutoff Ultracel® membrane
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
- Desalting, buffer exchange, and protein dialysis
Features and Benefits
- Ultracel® regenerated cellulose membrane with 30 kDa NMWCO delivers retentate recovery of greater than 90%
- Concentrates 500 μL down to 15 μL
- Concentration factor 25x to 30x
- High sample recovery (greater than 90% of dilute starting solution)
- Fast processing time: 10 to 30 minutes
- Reverse spin capability provides consistent recoveries
Physical form
Other Notes
Legal Information
저장 등급
11 - Combustible Solids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
프로토콜
Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.
If an antibody must be purified from a small sample volume (0.5-10 mL), then the Amicon® Pro Purification System is recommended.
문서
Filtration methods and membrane filter selection in nanoparticle purification and production.
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| UFC503024 | 08436037125867 |
| UFC503096 | 04053252464591 |
| UFC503008 | 04053252576331 |
| UFC5030BK | 04053252664656 |