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Merck

UFC203024

Amicon® Ultra Centrifugal Filter, 30 kDa MWCO

greener alternative

sample volume 2 mL, regenerated cellulose membrane, MWCO 30 kDa

동의어(들):

Centrifugal concentrator, 30 kDa MWCO, 2 mL sample volume

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UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
Material:
polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing
Pore size:
30 kDa MWCO
Working volume:
≤2 mL , 2 mL
Sterility:
non-sterile
Packaging:
pkg of 24 pkg
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Quality Level

material

polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing

sterility

non-sterile

product line

Amicon®

packaging

pkg of 24 pkg

manufacturer/tradename

Amicon® Ultra

sustainability

Greener Alternative Product

parameter

2 mL sample volume

technique(s)

protein extraction: suitable, protein purification: suitable

L

119.7 mm

diam.

15.9 mm

filtration area

1 cm2

working volume

≤2 mL , 2 mL

pore size

30 kDa MWCO

greener alternative category

shipped in

ambient

General description

Amicon® Ultra-2 mL centrifugal devices with Ultracel® 30,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, Ovalbumin (45 kDa) at 1 mg/ml initial concentration is typically retained at 97% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 50-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
The device can be spun in a swinging bucket or fixed angle rotor. Amicon® Ultra-2 mL devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 2 mL filters deliver the highest recovery with shortest spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from bio-circular material allocated using the ISCC mass balance approach. Click here for more information.

Application

  • Ultrafiltration with 30,000 Dalton molecular weight cutoff Ultracel® membrane.
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
  • Purification of macromolecular components found in tissue culture extracts or cell lysates.
  • Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.
  • Desalting, buffer exchange and protein dialysis.
  • Size exclusion and protein fractionation.

Features and Benefits

  • Ultracel® regenerated cellulose membrane with 30 kDa NMWCO delivers retentate recovery of greater than 90%
  • Concentrates 2 mL down to 15-70 μL
  • 98% sample recovery
  • Fast processing time: 10- to -30-minute spin times
  • Engineered dead stop
  • Reverse spin capability provides consistent recoveries

Physical form

Color Code: Clear

Other Notes

Replaces: 4208

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany


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문서 라이브러리 방문


문서

Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.

관련 콘텐츠

"The Amicon® Ultra 2 mL filter provides a fast and easy way to concentrate, desalt and exchange the buffer for both proteins and nucleic acids. Now you can concentrate 2 mL down to 15 – 70 μL in an Amicon® Ultra centrifugal device."


Sergey A Dyshlovoy et al.
Marine drugs, 18(5) (2020-05-15)
The phenomenon of high sugar consumption by tumor cells is known as Warburg effect. It results from a high glycolysis rate, used by tumors as preferred metabolic pathway even in aerobic conditions. Targeting the Warburg effect to specifically deliver sugar



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SKUGTIN
UFC20302404053252691799