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PC177L

Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb

Name: Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb
Brand: MM

lyophilized, Calbiochem^®

동의어(들):

Rabbit Anti-Serotonin Transporter, Serotonin Transporter Antibody

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제품정보 (DICE 배송 시 비용 별도)

NACRES:

NA.43

UNSPSC Code:

12352203

Clone:

polyclonal

Species reactivity:

mouse, rat

Application:

—

Citations:

기술 서비스

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도움 문의

속성

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized

contains

≤0.1% sodium azide as preservative

species reactivity

mouse, rat

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(Frozen Sections (1:10,000-1:15,000;
Immunoblotting
Immunofluorescence (1:200-1:400))

isotype

IgG

shipped in

ambient

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Slc6A4(15567)

설명

General description

Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the serotonin (5-HT) transporter protein.

Recognizes the serotonin (5-HT) transporter in hypothalamus raphe nuclei and spinal cord.

This Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence for the detection of Serotonin (5-HT) Transporter (602-622).

Immunogen

Rat

a synthetic peptide corresponding to amino acids 602-622 of rat 5-HT transporter

Application

Frozen Sections (1:10,000-1:15,000; see application references)
Immunoblotting (see comments)
Immunofluorescence (1:200-1:400)

Physical form

Undiluted serum.

Preparation Note

Reconstitute the lyophilized antibody with 100 µl of sterile distilled H₂O. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Further dilute with sterile PBS or Tris buffer to dilutions no higher than 1:10.

Analysis Note

Positive Control
Rat naphe nuclei, hypothalamus, cortex, or spinal cord

Other Notes

Antibody specificity was examined in the rat cortex, hypothalamus, raphe nuclei, and spinal cord. Staining is completely eliminated by pretreatment of antibody with Serotonin (5-HT) Transporter Control Peptide (Cat. No. PP87) at a concentration of 5 µg/ml. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. The specificity of this antibody was also determined by immunoblotting analysis using rat brain extracts of the cortex, hypothalamus, midbrain, and hindbrain, which specifically detected a single band. Antibody should be titrated for optimal results in individual systems.

Zhou, F.C., et al. 1996. Mol. Brain Res.43, 267.
Blakely, R.D., et al. 1994. J. Exp. Biol.196, 263.
Coccaro, E.F. and Murphy, D.L. (Eds.) 1990. in Serotonin in Major Disorders. The Progress in Psychiatry Series, American Psychiatric Press, Washington, D.C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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저장 등급

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c