Anti-Integrin αV (ITGAV/CD51) Antibody

Epitope: extracellular, azide free

Human integrin alphaVbeta3 heterodimer isolated from placenta.

Anti-Integrin αV Antibody, extracellular, clone AV1, azide free detects level of Integrin αV & has been published & validated for use in FC & WB.

Flow cytometry: 1:5-1:50

Western Blot. Under non-reducing conditions recognizes a band at approximately 165 kDa corresponding to integrin alphaV. Does not recognize the reduced protein.

Function-blocking of integrin alphaV-mediated adhesion to ECM proteins. (1:5-1:20, in HEPES buffer pH 7.4 containing 1mM Mn++ and 1mM Mg++).

Optimal working dilutions and protocols must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

Specific for human integrin alphaV (other species not tested).

Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ micron membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.