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Merck

EZHIL8

Human IL-8 ELISA Kit

detect IL-8 in 50 μL serum, plasma or cell culture supernatent

동의어(들):

Granulocyte chemotactic protein 1, IL-8, Interleukin-8, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, T-cell chemotactic factor

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
41116158
NACRES:
NA.84
Gene information:
human ... CXCL8(3576)
Input:
sample type plasma (K2 EDTA)
sample type cell culture supernatant
sample type serum
Species reactivity:
human
Storage temp.:
2-8°C
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제품 이름

Human IL-8 Conferma® ELISA, quantification of Human IL-8 in serum, plasma, or culture media samples

Quality Level

species reactivity

human

parameter

50 μL sample volume (4hr assay)

technique(s)

ELISA: suitable

input

sample type plasma (K2 EDTA)
sample type cell culture supernatant
sample type serum

UniProt accession no.

application(s)

research use

assay range

accuracy: 77-102 (plasma)%
linearity: 105 (plasma)%, inter-assay cv: <4.7%
intra-assay cv: <4.0%
standard curve range: 0.82-200 pg/mL
accuracy: 70-105 (serum)%
linearity: 94-106 (serum)%

detection method

colorimetric (450nm/590nm)

storage temp.

2-8°C

Gene Information

human ... CXCL8(3576)

General description

IL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. IL-8 also participates with other cytokines in the proinflammatory signaling cascade. The protein is implicated in numerous pathologies including, bacterial, cancer, cardiovascular and viral diseases.
IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

This is a 96 well format sandwich ELISA which uses our “Conferma” process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots.

Application

This Human Interleukin-8 (IL-8) ELISA kit is used for the quantification of Human IL-8 in serum, plasma, or culture media samples.

Analytes available:
  • IL-8

Research Category:
  • Cytokines
  • Inflammation & Immunology
  • Immune Response
  • Metabolic Disease

Analysis Note

3 hour 45 minute assay, room temperature, 50μl of neat serum or plasma is recommened.

Legal Information

CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For research use only. Not for use in diagnostic procedures.




시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문


프로토콜

This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.

이 문서에서는 가장 많이 알려진 4가지 ELISA 프로토콜에 대해 설명합니다. 여기에는 샌드위치 ELISA 프로토콜, 인산화 분석법, EIA 분석법 및 세포 기반 분석법이 있습니다.

문서

Learn about the Conferma® ELISA development and manufacturing methods that provide strong sample detection and long-term assay and lot consistency, giving you confidence in your research.

강력한 시료 검출 및 일관된 로트의 제품으로 장기간 분석을 가능하게 하여 연구에 확신을 가져다주는 Conferma® ELISA 개발 및 제조 방법에 대해 알아보세요.

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