Human Integrin αVβ3, Octyl-β-D-glucopyranoside Formulation

Integrin alphaVbeta3 was purified from human placenta by affinity chromatography using immobilized monoclonal antibodies to alphaVbeta3 integrin. A tissue detergent extract, which was applied to the column was prepared as previously described [Belkin, 1990]. Product was tested and found negative for HIV and Hepatitis B.

Electrophoresis and Immunoblotting control. Also useful for in vitro binding experiments with metalloproteinase MMP-2 .

Purified protein inTris buffered saline with 100 mM Octyl-beta-D-glucopyranoside, and sodium azide. This format of alphaVbeta3 is appropriate for functional evaluations.

Maintain at -70°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/ thaw cycles.

Specific Activity: Confirmed by vWf (von Willibrand factor) binding assay. It is expected that this preparation of alphaVbeta3 integrin will also interact with other alphaVbeta3 binding proteins in an RGD-dependent fashion.

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