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BugBuster® Master Mix
동의어(들):
BugBuster® Protein Extraction Reagent with Benzonase® Nuclease and rLysozyme solution, Cell Lysis Master Mix
일반 설명
The BugBuster® Master Mix enables efficient protein purification and other related applications. It replaces the need for mechanical methods like the French press or sonication. The two available package sizes contain sufficient reagents for extracting proteins from either 20 grams or 100 grams of cell paste.
애플리케이션
BugBuster® Master Mix has been used:
- to resuspend and lyse the cell pellets during protein purification
- to extract proteins for immunoblotting
- as a bacterial lysis buffer for the preparation of inclusion bodies
생화학적/생리학적 작용
BugBuster® Protein Extraction Reagent is specifically designed to gently disrupt the cell wall of E. coli, allowing the release of soluble proteins. This unique formulation utilizes a detergent mix that can effectively perforate the cell wall without denaturing the soluble proteins. By combining BugBuster Reagent, rLysozyme solution, and Benzonase Nuclease, our BugBuster Master Mix enhances protein extraction efficiency and simplifies downstream processing of protein extracts.
특징 및 장점
- Efficient lysis of Gram-positive and Gram-negative bacteria
- Maximum recovery of active, soluble protein
- Capable of cell wall perforation without denaturing soluble protein
- Useful for the preparation of high purity inclusion bodies
- Compatible with protease and phosphatase inhibitor cocktail
- Offers a convenient, fast, and cost-effective solution for releasing the expressed target protein.
- The Master Mix eliminates the need to dilute or perform separate addition steps.
법적 정보
BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Toxicity: Standard Handling (A)
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Matthew Thomas Doyle et al.
Molecular cell, 81(9), 2000-2012 (2021-03-12)
The β-barrel assembly machine (BAM) integrates β-barrel proteins into the outer membrane (OM) of Gram-negative bacteria. An essential BAM subunit (BamA) catalyzes integration by promoting the formation of a hybrid-barrel intermediate state between its own β-barrel domain and that of
Daniel G Isom et al.
Proteins, 79(4), 1034-1047 (2011-03-10)
Protein thermodynamic stability is a fundamental physical characteristic that determines biological function. Furthermore, alteration of thermodynamic stability by macromolecular interactions or biochemical modifications is a powerful tool for assessing the relationship between protein structure, stability, and biological function. High-throughput approaches
Yong Y Peng et al.
Bioengineered, 5(6), 378-385 (2014-12-09)
The collagen like domain Scl2 from Streptococcus pyogenes has been proposed as a potential biomedical material. It is non-cytotoxic and non-immunogenic and can be prepared in good yield in fermentation. The Scl2 collagen domain is about a quarter of the
Nicolas Broguiere et al.
Acta biomaterialia, 77, 182-190 (2018-07-15)
The bacterial ligase Sortase A (SA) and its mutated variants have become increasingly popular over the last years for post-translational protein modifications due to their unparalleled specificity and efficiency. The aim of this work was to study SA as a
Shmuel Gleizer et al.
Cell, 179(6), 1255-1263 (2019-11-30)
The living world is largely divided into autotrophs that convert CO2 into biomass and heterotrophs that consume organic compounds. In spite of widespread interest in renewable energy storage and more sustainable food production, the engineering of industrially relevant heterotrophic model
문서
Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens
관련 콘텐츠
Read an automated protocol for protein purification using PureProteome™ nickel magnetic beads on the AAW™ automated assay workstation and see results comparing manual vs automated runs.
세포 용해 및 단백질 추출 방법은 세제 용해부터 기계적 파쇄법에 이르기까지 다양한 기술을 포함하며 연구에 필요한 요구 사항을 지원합니다.
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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