Fractogel^® EMD SO₃ ⁻ (M) Resin

5 x 10 mm

RoboColumn Fractogel^® SO3 (M), 0.2 ml enables:

• Increased binding strength at low pH
• High aggregate removal efficiency combined with high dynamic binding capacity
• Lower elution volumes for the highest purity levels
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel^® EMD SO₃^- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany