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Merck

1.08178

Trifluoroacetic acid

for protein sequence analysis

동의어(들):

TFA

조직 및 계약 가격을 보려면 로그인를 클릭합니다.

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제품정보 (DICE 배송 시 비용 별도)

Linear Formula:
CF3COOH
CAS 번호:
Molecular Weight:
114.02
UNSPSC Code:
12352106
NACRES:
NA.21
EC Index Number:
200-929-3
MDL number:
Beilstein/REAXYS Number:
742035
Assay:
≥99.7% (acidimetric)
Form:
liquid
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vapor density

3.9 (vs air)

Quality Level

vapor pressure

97.5 mmHg ( 20 °C)

assay

≥99.7% (acidimetric)

form

liquid

impurities

≤0.02% Water (Karl Fischer)

refractive index

n20/D 1.3 (lit.)

pH

1 (10 g/L in H2O)

mp

−15.4 °C (lit.)

solubility

soluble 10 g/mL

density

1.489 g/mL at 20 °C (lit.)

storage temp.

2-30°C

SMILES string

OC(C(F)(F)F)=O

InChI

1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7)

InChI key

DTQVDTLACAAQTR-UHFFFAOYSA-N

Application

Trifluoroacetic acid (TFA) can be used as an ion-pairing agent in a reverse-phase HPLC system to separate peptides because it enhances peak resolution. It also has low absorption detection wavelengths and is relatively volatile and can be easily removed. For the majority of peptide separations, TFA has been used at concentrations ranging from 0.05 to 0.1%(6.5-13 mM).

Analysis Note

Assay (acidimetric): ≥ 99.7 %
Identity (IR-spectrum): passes test
Density (d 20 °C/20 °C): about 1.53
Boiling point: about 72 °C
Water (according to Karl Fischer): ≤ 0.02 %


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Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1A

wgk

WGK 2

저장 등급

8A - Combustible corrosive hazardous materials



시험 성적서(COA)

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문서 라이브러리 방문



Y Chen et al.
Journal of chromatography. A, 1043(1), 9-18 (2004-08-20)
Trifluoroacetic acid (TFA) remains the dominant mobile phase additive for reversed-phase high-performance liquid chromatography (RP-HPLC) of peptides after more than two decades since its introduction to this field. Generally, TFA has been employed in a concentration range of 0.05-0.1% (6.5-13



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