T9285
Tampon Tris-EDTA
100X concentrate for Northern and Southern blotting
Synonyme(s) :
Tampon TE solution
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Molecular Biology
Quality Level
sterility
0.2 μm filtered
form
liquid
pH
7.8-8.2 (1 × in water)
application(s)
sample preparation
foreign activity
DNase, RNase, none detected
storage temp.
room temp
SMILES string
NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
InChI key
VLEIUWBSEKKKFX-UHFFFAOYSA-N
General description
Tris EDTA buffer is commonly used in molecular biology for reconstitution and storage of many precipitated biomolecules, including DNA and RNA. Tris-HCl is a commonly used buffer and EDTA is a metal chelating agent. TE buffer solubilizes nucleic acids while protecting the molecules from degradation. It is used in nucleic acid isolation, which may be done prior to nucleic acid gel electrophoresis and Northern or Southern blot hybridization.
Application
TE buffer has been used for:
- isolate, resuspend, or store DNA and RNA for PCR or qPCR
- gel electrophoresis, including Northern and Southern blotting
- bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells
- antigen retrieval for immunohistochemistry
Preparation Note
TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.
Other Notes
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Classe de stockage
12 - Non Combustible Liquids
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nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Marika Bogdani et al.
Scientific reports, 7(1), 17231-17231 (2017-12-10)
Cystic fibrosis (CF)-related diabetes (CFRD) is thought to result from beta-cell injury due in part to pancreas exocrine damage and lipofibrosis. CFRD pancreata exhibit reduced islet density and altered cellular composition. To investigate a possible etiology, we tested the hypothesis
Detection of vaccinia virus, herpes simplex virus, varicella-zoster virus, and Bacillus anthracis DNA by LightCycler polymerase chain reaction after autoclaving: implications for biosafety of bioterrorism agents.
Espy MJ, et al.
Mayo Clinic Proceedings. Mayo Clinic, 77, 624-628 (2002)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, B-B (1989)
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| T9285-100ML | 04061838260468 |