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S6639

SSC Buffer 20× Concentrate

for Northern and Southern blotting, solution

Synonyme(s) :

20X SSC Buffer, SSC hybridization buffer, Saline-sodium citrate buffer

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A propos de cet article

NACRES:
NA.25
UNSPSC Code:
41105319
Service technique
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grade

Molecular Biology

Quality Level

200

description

Saline-Sodium Citrate buffer, made with ultrapure water

sterility

0.2 μm filtered

form

solution

color

colorless

pH

6.9 —7.1

application(s)

diagnostic assay manufacturing

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

storage temp.

room temp

General description

SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization.

Application

SSC Buffer 20× Concentrate has been used
  • as a hybridization buffer on in situ hybridization human-specific repetitive Alu sequence
  • as a washing buffer for membrane for Southern hybridization, dot blot of mice genomic DNA and northern blotting of microRNA
  • as an incubation buffer in fluorescence in situ hybridization (FISH) of L1 retrotransposon

Features and Benefits

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations

Preparation Note

SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).

Classe de stockage

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
118K8403
048K8413
097K8402
057K8404
037K8401

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Protocoles

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Articles

Fluorescent in situ Hybridization (FISH)

Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

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Liu H, et al.
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L1 retrotransposon expression in circulating tumor cells
Papasotiriou I, et al.
Testing, 12(2), e0171466-e0171466 (2017)
Michaela Asp et al.
Scientific reports, 7(1), 12941-12941 (2017-10-13)
Heart failure is a major health problem linked to poor quality of life and high mortality rates. Hence, novel biomarkers, such as fetal marker genes with low expression levels, could potentially differentiate disease states in order to improve therapy. In
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Numéro d'article de commerce international

RéférenceGTIN
S6639-1L04061838258816