S8140
SSPE Buffer
for Northern and Southern blotting, powder blend
Synonyme(s) :
Saline-Sodium Phosphate-EDTA Buffer
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A propos de cet article
NACRES:
NA.25
UNSPSC Code:
41105319
Service technique
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Molecular Biology
Quality Level
form
powder blend
pH
7.2-7.6 (20 × in water, high purity, ambient)
solubility
water, high purity: 20 ×, clear, colorless
foreign activity
DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected
storage temp.
room temp
General description
SSPE Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. This powder should be reconstituted to the indicated volume with molecular biology grade water before use. The resulting 20x liquid can be used straight or diluted as needed.
Application
- Influence of Saline Buffers over the Stability of High-Annealed Gold Nanoparticles Formed on Coverslips for Biological and Chemosensing Applications.: This study explores the role of saline buffers in maintaining the stability of high-annealed gold nanoparticles used in biological and chemosensing applications. The findings highlight the potential for enhanced sensor performance in detecting biological molecules (Zhou & Ionescu, 2020).
- Quantification of Surface Etching by Common Buffers and Implications on the Accuracy of Label-Free Biological Assays.: This research assesses the impact of various buffers, including SSPE buffer, on the surface etching of substrates used in label-free biological assays. The results indicate that buffer-induced surface changes can significantly affect assay accuracy (Ahn et al., 2012).
Features and Benefits
- Easy to reconstitute powder
- Multi-purpose use in hybridizations
Preparation Note
Final SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).
Classe de stockage
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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Protocoles
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Microfluidic-based enzymatic on-chip labeling of miRNAs
Sonja Vorwek
New Biotechnology (2008)
3'-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures
Igor V. Kutyavin
Nucleic Acids Research, 28(2) (2000)
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| S8140-1L | 04061838061102 |
| S8140-100ML | 04061835509591 |