KCQS02
KiCqStart® SYBR® Green qPCR ReadyMix™
with ROX™ for ABI instruments
Synonyme(s) :
qPCR master mix, sybr green qPCR
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A propos de cet article
form
liquid
usage
sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions
feature
dNTPs included, hotstart
storage condition
protect from light
technique(s)
RT-qPCR: suitable, qPCR: suitable
color
colorless
input
purified DNA
compatibility
for use with ABI 5700, for use with ABI 7000, for use with ABI 7300, for use with ABI 7700, for use with ABI 7900 HT Fast, for use with ABI 7900 HT, for use with ABI 7900, for use with ABI StepOne, for use with ABI StepOnePlus
detection method
SYBR® Green
shipped in
dry ice
storage temp.
−20°C
General description
Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.
Application
- in the amplification and quantification of cDNA reverse transcribed from RNA extracted from mice brain samples in a 2-step RT-qPCR assay
- to analyze DNA purified by ChIP technique
- to perform gene expression analysis
- in amplification of RNA isolated from hearts of adult TL wild-type fish by quantitative real-time polymerase chain reaction (PCR)
- Gene expression
- DNA quantification
- CHiP
Features and Benefits
- Assay results in as little as 33 minutes
- Highly efficient and sensitive real-time PCR results
- Little/no optimization required
Other Notes
packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.
Legal Information
Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocoles
Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.
Gradient PCR optimizes assay conditions by testing fixed primer concentrations across various annealing temperatures.
Optimization of qPCR conditions is important for the development of a robust assay. The two main approaches are optimization of primer concentration and/or annealing temperatures.
Articles
The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension
After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.
Contenu apparenté
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| KCQS02-250RXN | 04061835573332 |
| KCQS02-SAMPLE | 04061838099488 |
| KCQS02-1250RXN | 04061835573325 |
| KCQS02-5000RXN | 04061835573349 |