G7654
Gel Loading Solution
for NA electrophoresis, solution
Synonyme(s) :
DNA Gel Loading Solution, Gel Loading Buffer
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A propos de cet article
NACRES:
NA.25
UNSPSC Code:
12161703
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Laissez-nous vous aiderGeneral description
Gel loading solution is used as a tracking dye during electrophoresis. The dyes have a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:6 with sample before loading.
Application
Suitable for use with agarose or non-denaturing polyacrylamide gel electrophoresis (PAGE), which may be part of Northern and Southern blot hybridization procedures.
Preparation Note
Gel loading buffer contains 0.25% bromophenol blue, 0.25% xylene cyanol, and 40% sucrose.
Other Notes
Band migration can be expected as follows:
On polyacrylamide gels, xylene cyanole comigrates with approximately 450-460 bp DNA, while bromophenol blue comigrates with 15-100 bp DNA. On 0.5 – 1.4% agarose gels, xylene cyanole comigrates with 4 kb dsDNA, while bromophenol blue comigrates with 300 bp dsDNA.
On polyacrylamide gels, xylene cyanole comigrates with approximately 450-460 bp DNA, while bromophenol blue comigrates with 15-100 bp DNA. On 0.5 – 1.4% agarose gels, xylene cyanole comigrates with 4 kb dsDNA, while bromophenol blue comigrates with 300 bp dsDNA.
Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenu apparenté
David R Macinga et al.
Antimicrobial agents and chemotherapy, 47(8), 2526-2537 (2003-07-25)
We have characterized an early series of 5,6-bridged dioxinoquinolones which behaved strikingly different from typical quinolones. The 5,6-bridged dioxinoquinolones inhibited Escherichia coli DNA gyrase supercoiling activity but, unlike typical quinolones, failed to stimulate gyrase-dependent cleavable complex formation. Analogous unsubstituted compounds
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 6-6 (1989)
M K Bolla et al.
Journal of lipid research, 40(12), 2340-2345 (1999-12-10)
The apoE gene exhibits two common polymorphisms that have been associated with both coronary artery disease and Alzheimer's disease. The polymorphisms create the three allelic isoforms E2, E3, and E4 which are encoded by Cys;-Cys, Cys;-Arg, and Arg;-Arg at amino
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| G7654-5ML | 04061838099358 |