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B6185

Bionic™ Buffer, 10X Concentrate

for Northern and Southern blotting, solution

Synonyme(s) :

Sodium Borate Electrophoresis Buffer

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A propos de cet article

NACRES:
NA.25
UNSPSC Code:
41105319
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grade

Molecular Biology

Quality Level

200

description

Mode of use Voltage of 150 V is recommended for small electrophoresis chambers while a mid to large apparatus may be run at 200-250 V. User should test their chamber to determine optimum voltage.

form

solution

concentration

10 ×

impurities

DNase, RNase and nickase, none detected

pH

8.0-8.5 (20-25 °C)

storage temp.

room temp

General description

Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for higher voltage runs, extremely rapid running times, and high-resolution band separation.

Application

Suitable for:
  • Use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures
  • Use with TBA and TAE gels
  • Preparation of agarose gels

Features and Benefits

  • Faster run times
  • Sharper bands
  • Easy to dilute and use
  • Flexible use with a variety of gel types

Packaging

The 4 L and 10 L sizes are supplied in dispensers with a spigot.

Legal Information

Bionic is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

Test electrophoresis chambers to determine highest voltage. Voltages beyond the recommended range for your apparatus may result in blown fuses or damage to your apparatus.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B

Classe de stockage

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
035K61431
105K6013
104K6253
035K6143
085K6092

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Protocoles

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Carmen Naturil-Alfonso et al.
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Parthenogenetic embryos are one attractive alternative as a source of embryonic stem cells, although many aspects related to the biology of parthenogenetic embryos and parthenogenetically derived cell lines still need to be elucidated. The present work was conducted to investigate
Fast short-fragment PCR for rapid and sensitive detection of shrimp viruses.
Mrotzek G, et al.
Journal of Virological Methods, 168, 262-266 (2010)
Maria Desemparats Saenz-de-Juano et al.
Biology of reproduction, 87(4), 91-91 (2012-08-03)
Studies of embryo cryopreservation efficiency have focused mainly on technical and embryo factors. To determine how a slow freezing process affects embryo and fetal development, we studied in vivo development ability after the freezing procedure by assessing blastocyst development at

Numéro d'article de commerce international

RéférenceGTIN
B6185-100ML04061833434512
B6185-10L04061838177612
B6185-1L04061833434529
B6185-4L04061833434536