15356
Blotting-Nylon 66 membranes, type B, positive
for Northern and Southern blotting
Synonyme(s) :
Nylon 66-Blotting membranes, type B
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A propos de cet article
NACRES:
NA.25
UNSPSC Code:
41105339
Material:
Nylon
Service technique
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Nylon
Quality Level
grade
Molecular Biology
form
roll
surface coverage
surface (quaternary ammonium groups) ≥99%
pore size
0.45 μm pore size
storage temp.
room temp
General description
Blotting nylon provided on a roll is a standard item used in transfer of protein or nucleic acids during Western, Southern and Northern procedures. This membrane is positively charged nylon cast on a polyester support. It comes as a roll sized 0.2m x 3m, and can be cut to any size.
Application
Suitable for:
Protein transfer
Radioactive detection of blotted proteins and nucleic acids
Sensitive dot and slot blots of nucleic acids
Protein transfer
Radioactive detection of blotted proteins and nucleic acids
Sensitive dot and slot blots of nucleic acids
for high-sensitive nucleic acid blotting, dot and lot blots. Particularly suitable for radioactive detection. Suitable for protein transfer and immunoblotting.
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocoles
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Gerardas Dambrauskas et al.
Journal of experimental botany, 54(383), 699-706 (2003-01-30)
Very little is known about the expression patterns in plants of genes that encode proteins involved in the initiation of DNA replication. Partial cDNA sequences that encode Cdc6 and Mcm3 in tobacco have been isolated. The sequences were used as
K Pfeifer et al.
Journal of cell science, 106 ( Pt 2), 545-553 (1993-10-01)
Ubiquitination of proteins is a critical step in the controlled degradation process of many polypeptides. Here we show that sponges, the simplest multicellular group of eukaryotic organisms, are also equipped with the ubiquitin pathway. The polyubiquitin cDNA was isolated and
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| 15356-1EA | 04061835570317 |