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MilliporeSigma

G004619

HPLC Analysis of Bupivacaine Enantiomers on Astec® CHIROBIOTIC® V2, Formate Mobile Phase

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: CHIROBIOTIC V2, 25 cm x 4.6 mm I.D., 5 μm particles (15024AST)
mobile phase: [A] 20 mM ammonium formate, pH 4.1; [B] methanol; (5:95, A:B)
flow rate: 1 mL/min
column temp.: 20 °C
detector: UV, 220 nm
injection: 5 μL
sample: 1 mg/mL in methanol

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Analyte

Description
    Bupivacaine hydrochloride monohydrate, analytical standard, for drug analysis

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