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66828-U

BIOshell™ A400 Protein C4 (3.4 μm) HPLC Columns

L × I.D. 10 cm × 4.6 mm, HPLC Column

Synonym(s):

Superficially Porous Wide Pore C4 UHPLC Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52
L × i.d.:
10 cm × 4.6 mm
Particle size:
3.4 μm
Matrix active group:
C4 (butyl) phase
Pore size:
400 Å
Matrix:
spherical silica particle platform, superficially porous particle
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Product Name

BIOshell™ A400 Protein C4, 3.4 μm HPLC Column, 3.4 μm particle size, L × I.D. 10 cm × 4.6 mm

material

stainless steel hardware

Quality Level

100

agency

suitable for USP L26

description

Shell thickness (0.2 μm), Solid Core (3.0 μm)

product line

BIOshell™

feature

endcapped

manufacturer/tradename

BIOshell™

packaging

1 ea of

extent of labeling

0.4% carbon loading

parameter

600 bar max. pressure, 90 °C max. temp.

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

L × I.D.

10 cm × 4.6 mm

surface area

15 m2/g

surface coverage

4.2 μmol/m2

matrix

spherical silica particle platform, superficially porous particle

matrix active group

C4 (butyl) phase

particle size

3.4 μm

pore size

400 Å

operating pH range

2-9

separation technique

reversed phase

General description

BIOshell™ 400 Å Protein C4 columns contain 3.4 μm advanced Fused-Core particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns provide excellent separations on very hydrophobic proteins such as those found in the cell membrane. The C4 ligand combined with an exhaustively endcapped surface ensures optimum protein recovery of larger or very hydrophobic biomolecules. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 Å fully porous C4 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within the pH range of 2 to 9.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

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Articles

BIOshell™ A400 Protein C4 U/HPLC Columns

BIOshell™ A400 Protein C4 columns contain 3.4 μm particles with 400 Å pores, which are derivatized with dimethylbutyl silane and exhaustively endcapped for optimum protein recovery.

Global Trade Item Number

SKUGTIN
66828-U04061838550927