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About This Item
eCl@ss:
32119290
UNSPSC Code:
41115710
NACRES:
SB.54
material
fused silica
agency
AOAC® 2012.13,Ce 1-h05,996.06, meets requirements for USP G5
specific analyte(s)
benzene
parameter
≤25-250 °C temperature (isothermal or programmed)
Beta value
313
df
0.20 μm
technique(s)
gas chromatography (GC): suitable
L × I.D.
100 m × 0.25 mm
matrix active group
Non-bonded; poly(biscyanopropyl siloxane) phase, SP 2560
application(s)
agriculture
clinical
food and beverages
column type
capillary highly polar
General description
Application: This highly polar biscyanopropyl column was specifically designed for detailed separation of geometricpositional (cis/trans) isomers of fatty acid methyl esters (FAMEs). It is extremely effective for FAME isomer applications.
USP Code: This column meets USP G5 requirements.
Phase:
USP Code: This column meets USP G5 requirements.
Phase:
- Non-bonded
- Poly(biscyanopropyl siloxane)
- Subambient to 250 °C (isothermal or programmed)
Application
The SP-2560 Capillary GC Columns meets the requirements as a USP G5 and AOAC® 2012.13,Ce 1-h05,996.06 designation. They are specifically designed for the detailed separation of geometric and positional (cis/trans) isomers of fatty acid methyl esters (FAMEs) in array of industries.
Select Applicatons:
Select Applicatons:
- Comparision study for the simultaneous resolution of all the fatty acids (cis/trans) generally found in partially hydrogenated vegetable oils.
- Utilization in GCxGC for separation of trans fats including elaidic and vaccenic acids in several foods including butter, beef tallow, cheese, and milk.
Other Notes
We offer a variety of chromatography accessories including analytical syringes
Legal Information
AOAC is a registered trademark of AOAC International
SP is a registered trademark of Sigma-Aldrich Co. LLC
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Protocols
FAME Standard for Optimizing GC System Performance
A protocol for GC Analysis of a 37-Component FAME Mix on SP™-2560.
Fast GC Analysis of Detailed cis/trans Fatty Acid Methyl Esters (FAMEs) on the 75 m SP™-2560 Capillary Column
Fatty acid composition of human erythrocyte membranes by capillary gas chromatography-mass spectrometry.
Alexander LR
Journal of Chromatography. B, Biomedical Applications, 342, 1-12 (1985)
Evaluation of the CP-Sil 88 and SP-2560 GC columns used in the recently approved AOCS official method Ce 1h-05: Determination of cis-, trans-, saturated, monounsaturated, and polyunsaturated fatty acids in vegetable or non-ruminant animal oils and fats by capillary GLC method.
Ratnayake WMN
Journal of the American Oil Chemists' Society, 83 (6), 475-488 (2006)
Antonio Natalello et al.
Journal of agricultural and food chemistry, 67(16), 4472-4482 (2019-04-02)
This work investigated the effects of feeding whole pomegranate byproduct (WPB) to lambs on ruminal, liver, and intramuscular fatty acids (FA). Seventeen lambs, divided into two groups, were fed for 36 days with a cereal-based concentrate diet (CON) or with
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 24056 | 04061835551583 |